Institute of Molecular Biosciences, Mahidol University, Salaya Campus, 25/25 Putthamonthol Road 4, Salaya, Nakhon Pathom 73170, Thailand.
Biochem J. 2012 Feb 15;442(1):181-90. doi: 10.1042/BJ20111747.
The cytosolic GST (glutathione transferase) superfamily has been annotated in the Drosophila melanogaster genome database. Of 36 genes, four undergo alternative splicing to yield a total of 41 GST proteins. In the present study, we have obtained the 41 transcripts encoding proteins by RT (reverse transcription)-PCR using RNA template from Drosophila S2 cells, an embryonic cell line. This observation suggests that all of the annotated DmGSTs (D. melanogaster GSTs) in the proteome are expressed in the late embryonic stages of D. melanogaster. To avoid confusion in naming these numerous DmGSTs, we have designated them following the universal GST nomenclature as well as previous designations that fit within this classification. Furthermore, in the cell line, we identified an apparent processed pseudogene, gste8, in addition to two isoforms from the Delta class that have been published previously. Only approximately one-third of the expressed DmGSTs could be purified by conventional GSH affinity chromatography. The diverse kinetic properties as well as physiological substrate specificity of the DmGSTs are such that each individual enzyme displayed a unique character even compared with members from the same class.
细胞质 GST（谷胱甘肽转移酶）超家族已在黑腹果蝇基因组数据库中进行了注释。在 36 个基因中，有 4 个通过选择性剪接产生总共 41 种 GST 蛋白。在本研究中，我们使用来自果蝇 S2 细胞（一种胚胎细胞系）的 RNA 模板通过 RT（反转录）-PCR 获得了编码蛋白质的 41 个转录本。这一观察结果表明，在黑腹果蝇胚胎发育的晚期，蛋白质组中所有注释的 DmGSTs（黑腹果蝇 GSTs）都有表达。为了避免在命名这些大量的 DmGSTs 时产生混淆，我们按照通用 GST 命名法以及适合于该分类的先前命名法对其进行了命名。此外，在细胞系中，我们除了鉴定出以前发表的两个来自 Delta 类的同工体外，还鉴定出一个明显的加工假基因 gste8。只有大约三分之一的表达 DmGSTs 可以通过常规 GSH 亲和层析进行纯化。DmGSTs 的多样化动力学特性和生理底物特异性使得每个单独的酶即使与同一类别的成员相比也具有独特的特征。
