建立并应用高效液相色谱-串联质谱法分析中国肉类中的两种单端孢霉烯族化合物：脱氧雪腐镰刀菌烯醇和 T-2 毒素。
Development and application of a method for the analysis of two trichothecenes: deoxynivalenol and T-2 toxin in meat in China by HPLC-MS/MS.
机构信息
College of Food Science, Southwest University, Chongqing 400716, PR China.
出版信息
Meat Sci. 2012 Mar;90(3):613-7. doi: 10.1016/j.meatsci.2011.10.002. Epub 2011 Oct 12.
A reliable and sensitive method was developed and successfully applied for the determination of deoxynivalenol and T-2 toxin simultaneously in pig dorsal muscle, pig back fat and chicken muscle by high performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) analysis. Limit of detection of deoxynivalenol and T-2 was 0.02μg/kg and 0.007μg/kg, and limit of quantification of deoxynivalenol and T-2 was 0.07μg/kg and 0.02μg/kg, respectively. Sixty-six meat samples were analyzed and deoxynivalenol was detected in the samples of pig back fat, with concentrations lower than 0.5μg/kg, and T-2 toxin was detected in the samples of pig dorsal muscle, pig back fat and chicken muscle, with concentrations lower than 0.5μg/kg. The results of sample analysis show that only trace residues of deoxynivalenol and T-2 toxin were detected in the samples analyzed.
建立了一种可靠、灵敏的方法，采用高效液相色谱-串联质谱（HPLC-MS/MS）分析技术，成功地同时检测猪背肌、猪背脂和鸡肉中的脱氧雪腐镰刀菌烯醇和 T-2 毒素。脱氧雪腐镰刀菌烯醇和 T-2 毒素的检测限分别为 0.02μg/kg 和 0.007μg/kg，定量限分别为 0.07μg/kg 和 0.02μg/kg。分析了 66 个肉样，在猪背脂样品中检测到脱氧雪腐镰刀菌烯醇，浓度低于 0.5μg/kg，在猪背肌、猪背脂和鸡肉样品中检测到 T-2 毒素，浓度低于 0.5μg/kg。样品分析结果表明，在所分析的样品中仅检测到痕量的脱氧雪腐镰刀菌烯醇和 T-2 毒素残留。
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