Department of Experimental Physics, Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava, Slovakia.
J Mater Sci Mater Med. 2012 Jan;23(1):47-50. doi: 10.1007/s10856-011-4498-x. Epub 2011 Nov 18.
Micro-domains of modified surface potential (SP) were created on hydroxyapatite films by direct patterning by mid-energy focused electron beam, typically available as a microprobe of Scanning Electron Microscopes. The SP distribution of these patterns has been studied on sub-micrometer scale by the Kelvin Probe Force Microscopy method as well as lysozyme adsorption. Since the lysozyme is positively charged at physiological pH, it allows us to track positively and negatively charged areas of the SP patterns. Distribution of the adsorbed proteins over the domains was in good agreement with the observed SP patterns.
通过使用中能聚焦电子束直接对羟基磷灰石薄膜进行图案化，在其表面产生了具有改性表面电势（SP）的微域。这种中能聚焦电子束通常作为扫描电子显微镜的微探针使用。通过使用 Kelvin 探针力显微镜方法以及溶菌酶吸附实验，对这些图案的 SP 分布进行了亚微米级别的研究。由于溶菌酶在生理 pH 值下带正电荷，因此可以用它来追踪 SP 图案中的带正电荷和带负电荷区域。在这些区域上吸附的蛋白质分布与观察到的 SP 图案吻合较好。
