聚乙二醇胁迫对黄芩悬浮培养物中黄酮类化合物积累及相关基因表达的影响
[Effects of PEG stress on flavonoids accumulation and related gene expression in suspension of Scutellaria baicalensis].
作者信息
Yang Zhaochun, Yuan Yuan, Chen Min, Shuai Lingfei, Xiao Qian, Lin Shufang
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medicinal Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Aug;36(16):2157-61.
OBJECTIVE
To study the effects of PEG stress on baicalin, baicalein accumulation induced by an increased concentration of PEG solution and the related genes' expression in suspension of Scutellaria baicalensis.
METHOD
The content of baicalin, baicalein in suspension of S. baicalensis was determined by HPLC. The related genes' expression was analyzed by semi-quantitative PCR.
RESULT
The content of proline in suspension of S. baicalensis was promoted by PEG treatment. Ten percent PEG treatment promoted the expression of PAL and the content of baicalein in experimental material via a drought stress. 20% PEG solution treatment promoted the expression of UBGAT. At the same time, the increased activity of APX inhibited the progress of eliminating reactive oxygen by baicalein, which induced the transformation from baicalein to baicalin.
CONCLUSION
Active ingredient in suspension of S. baicalensis was promoted significantly via a stress of light concentration of PEG solution.
目的
研究聚乙二醇（PEG）胁迫对黄芩悬浮培养物中黄芩苷、黄芩素积累及相关基因表达的影响。
方法
采用高效液相色谱法测定黄芩悬浮培养物中黄芩苷、黄芩素的含量。通过半定量PCR分析相关基因的表达。
结果
PEG处理促进了黄芩悬浮培养物中脯氨酸的含量。10%PEG处理通过干旱胁迫促进了苯丙氨酸解氨酶（PAL）的表达及实验材料中黄芩素的含量。20%PEG溶液处理促进了尿苷二磷酸葡萄糖：黄酮糖基转移酶（UBGAT）的表达。同时，抗坏血酸过氧化物酶（APX）活性的增加抑制了黄芩素清除活性氧的进程，从而诱导黄芩素向黄芩苷转化。
结论
低浓度PEG溶液胁迫显著促进了黄芩悬浮培养物中活性成分的积累。
Zotero 插件