[光照对黄芩悬浮培养物中黄酮类化合物积累及相关基因表达的影响]
[Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis].
作者信息
Chen Shunqin, Yuan Yuan, Luo Yujiang, Huang Luqi, Chen Ping, Li Xiaoming
机构信息
Wuhan Polytechnic University, Wuhan 430023, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Mar;35(6):682-5.
OBJECTIVE
To investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.
METHOD
The content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR.
RESULT
Active ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995).
CONCLUSION
Active ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.
目的
研究光照对黄芩悬浮培养物中黄芩苷、黄芩素积累及相关基因表达的影响。
方法
采用高效液相色谱法测定黄芩悬浮培养物中黄芩苷、黄芩素的含量。通过半定量聚合酶链反应分析相关基因的表达。
结果
光照显著促进黄芩悬浮培养物中活性成分的积累，光照条件下苯丙氨酸解氨酶（PAL）基因转录水平高于黑暗条件，尿苷二磷酸葡萄糖醛酸转移酶（UBGAT）基因表达水平与黄芩素含量显著相关（r = -0.995）。
结论
光照显著促进黄芩悬浮培养物中活性成分及相关基因（PAL、UBGAT）的表达。
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