Su Hu, Song Shurui, Yan Xin, Fang Limin, Zeng Bin, Zhu Youlin
Life Science Department, Nanchang University, Nanchang, Jiangxi Province, China.
Life Science Department, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi Province, China.
PLoS One. 2018 Feb 13;13(2):e0192114. doi: 10.1371/journal.pone.0192114. eCollection 2018.
Salicylic acid (SA) is synthesized via the phenylalanine lyase (PAL) and isochorismate synthase (ICS) pathways and can influence the stress response in plants by regulating certain secondary metabolites. However, the association between SA and particular secondary metabolites in the Chinese medicinal plant Scutellaria baicalensis Georgi is unclear. To elucidate the association between SA and the secondary metabolites baicalin and baicalein, which constitute the primary effective components of S. baicalensis, we subjected seedlings to drought and salt stress and exogenous SA treatment in a laboratory setting and tested the expression of PAL and ICS, as well as the content of free SA (FSA), total SA (TSA), baicalin, and baicalein. We also assessed the correlation of FSA and TSA with PAL and ICS, and with baicalin and baicalein accumulation, respectively. The results indicated that both FSA and TSA were positively correlated with PAL, ICS, and baicalin, but negatively correlated with baicalein. The findings of this study improve our understanding of the manner in which SA regulates secondary metabolites in S. baicalensis.
水杨酸（SA）通过苯丙氨酸解氨酶（PAL）和异分支酸合成酶（ICS）途径合成，并且可以通过调节某些次生代谢产物来影响植物的应激反应。然而，药用植物黄芩中SA与特定次生代谢产物之间的关联尚不清楚。为了阐明SA与构成黄芩主要有效成分的次生代谢产物黄芩苷和黄芩素之间的关联，我们在实验室环境中对幼苗进行干旱和盐胁迫以及外源SA处理，并检测了PAL和ICS的表达，以及游离SA（FSA）、总SA（TSA）、黄芩苷和黄芩素的含量。我们还分别评估了FSA和TSA与PAL和ICS以及与黄芩苷和黄芩素积累的相关性。结果表明，FSA和TSA均与PAL、ICS和黄芩苷呈正相关，但与黄芩素呈负相关。本研究结果增进了我们对SA调节黄芩次生代谢产物方式的理解。
