[黄芩过氧化氢清除系统与黄酮类化合物积累的相关性]
[Correlation between H2O2 scavenging system and flavonoids accumulation of Scutellaria baicalensis].
作者信息
Shuai Lingfei, Yuan Yuan, Chen Ping, Lin Shufang
机构信息
Wuhan Polythechnic University, Wuhan 430023, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(13):1707-10.
OBJECTIVE
Study on correlation between H2O2 scavenging system and flavonoids accumulation of Scutellaria baicalensis.
METHOD
The content of baicalin and baicalein in suspension cell of S. baicalensis was determined by HPLC. The content of total flavonoids and H2O2, the activity of POD and PAL was detected by UV spectrophotometry.
RESULT
The content of total flavonoid and the activity of PAL increased significantly in 12 days after 40 degrees C, dark and PEG stress. Around 12 days after NPA, NPA +40 degrees C, 40 degrees C, NPA + dark, dark and PEG stress, the content of baicalin declined and the content of baicalein rise, the activity of POD showed an increasing trend, and level of H2O2 remain stable.
CONCLUSION
Moderate environmental stress could promote the accumulation of total flavonoids in S. baicalensis, baicalin convert to baicalein by POD, and maintaining the stability of H2O2 content to avoid oxidative damage.
目的
研究黄芩过氧化氢清除系统与黄酮类化合物积累之间的相关性。
方法
采用高效液相色谱法测定黄芩悬浮细胞中黄芩苷和黄芩素的含量。通过紫外分光光度法检测总黄酮含量、过氧化氢含量、过氧化物酶（POD）和苯丙氨酸解氨酶（PAL）的活性。
结果
40℃、黑暗和聚乙二醇（PEG）胁迫处理12天后，总黄酮含量和PAL活性显著增加。在NPA、NPA + 40℃、40℃、NPA + 黑暗、黑暗和PEG胁迫处理约12天后，黄芩苷含量下降，黄芩素含量上升，POD活性呈上升趋势，过氧化氢水平保持稳定。
结论
适度的环境胁迫可促进黄芩中总黄酮的积累，黄芩苷通过POD转化为黄芩素，并维持过氧化氢含量的稳定以避免氧化损伤。
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