Genome-Scale Biology Program, Institute of Biomedicine, University of Helsinki, Helsinki, Finland.
Nat Methods. 2011 Nov 20;9(1):72-4. doi: 10.1038/nmeth.1778.
Counting individual RNA or DNA molecules is difficult because they are hard to copy quantitatively for detection. To overcome this limitation, we applied unique molecular identifiers (UMIs), which make each molecule in a population distinct, to genome-scale human karyotyping and mRNA sequencing in Drosophila melanogaster. Use of this method can improve accuracy of almost any next-generation sequencing method, including chromatin immunoprecipitation-sequencing, genome assembly, diagnostics and manufacturing-process control and monitoring.
对单个 RNA 或 DNA 分子进行计数很困难，因为它们难以进行定量复制以进行检测。为了克服这一限制，我们在果蝇的人类基因组染色体组型分析和 mRNA 测序中应用了独特分子标识符 (UMI)，它使群体中的每个分子都具有独特性。该方法的使用可以提高几乎任何下一代测序方法的准确性，包括染色质免疫沉淀测序、基因组组装、诊断和制造过程控制和监测。
