来自腐败希瓦氏菌的低氧化还原电位四血红素细胞色素c3的纯化及性质
Purification and properties of a low-redox-potential tetraheme cytochrome c3 from Shewanella putrefaciens.
作者信息
Tsapin A I, Nealson K H, Meyers T, Cusanovich M A, Van Beuumen J, Crosby L D, Feinberg B A, Zhang C
机构信息
Center for Great Lakes Studies and Department of Chemistry, University of Wisconsin-Milwaukee, USA.
出版信息
J Bacteriol. 1996 Nov;178(21):6386-8. doi: 10.1128/jb.178.21.6386-6388.1996.
Abstract
Shewanella putrefaciens is a facultatively anaerobic bacterium in the gamma group of the proteobacteria, capable of utilizing a wide variety of anaerobic electron acceptors. An examination of its cytochrome content revealed the presence of a tetraheme, low-redox-potential (E'o = -233 mV), cytochrome c-type cytochrome with a molecular mass of 12,120 Da and a pI of 5.8. The electron spin resonance data indicate a bis-histidine coordination of heme groups. Reduction of ferric citrate was accompanied by oxidation of the cytochrome. The biochemical properties suggested that this protein was in the cytochrome c3 group, which is supported by N-terminal sequence data up to the first heme binding site.
摘要
腐败希瓦氏菌是变形菌γ群中的兼性厌氧菌，能够利用多种厌氧电子受体。对其细胞色素含量的检测显示存在一种四血红素、低氧化还原电位（E'o = -233 mV）、分子量为12,120 Da且pI为5.8的细胞色素c型细胞色素。电子自旋共振数据表明血红素基团为双组氨酸配位。柠檬酸铁的还原伴随着细胞色素的氧化。生化特性表明该蛋白质属于细胞色素c3组，这一点得到了直至第一个血红素结合位点的N端序列数据的支持。
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