Department of Orthopaedic Surgery, School of Medicine, Keio University, Shinjuku, Tokyo, Japan.
Infect Immun. 2012 Feb;80(2):733-41. doi: 10.1128/IAI.06166-11. Epub 2011 Nov 21.
Osteomyelitis remains a serious problem in the orthopedic field. There are only a few animal models in which the quantity and distribution of bacteria can be reproducibly traced. Here, we established a real-time quantitative mouse model of osteomyelitis using bioluminescence imaging (BLI) without sacrificing the animals. A bioluminescent strain of Staphylococcus aureus was inoculated into the femurs of mice. The bacterial photon intensity (PI) was then sequentially measured by BLI. Serological and histological analyses of the mice were performed. The mean PI peaked at 3 days, and stable signals were maintained for over 3 months after inoculation. The serum levels of interleukin-6, interleukin-1β, and C-reactive protein were significantly higher in the infected mice than in the control mice on day 7. The serum monocyte chemotactic protein 1 level was also significantly higher in the infected group at 12 h than in the control group. A significantly higher proportion of granulocytes was detected in the peripheral blood of the infected group after day 7. Additionally, both acute and chronic histological manifestations were observed in the infected group. This model is useful for elucidating the pathophysiology of both acute and chronic osteomyelitis and to assess the effects of novel antibiotics or antibacterial implants.
骨髓炎仍然是骨科领域的一个严重问题。只有少数动物模型可以可重复地追踪细菌的数量和分布。在这里，我们使用生物发光成像（BLI）建立了一种实时定量的骨髓炎小鼠模型，而无需牺牲动物。将发光的金黄色葡萄球菌菌株接种到小鼠的股骨中。然后通过 BLI 连续测量细菌的光子强度（PI）。对小鼠进行血清学和组织学分析。PI 的平均值在第 3 天达到峰值，接种后 3 个月以上仍保持稳定信号。与对照组相比，感染组小鼠在第 7 天的血清白细胞介素 6、白细胞介素 1β 和 C 反应蛋白水平显著升高。感染组在 12 小时时的血清单核细胞趋化蛋白 1 水平也明显高于对照组。在感染组，外周血中的粒细胞比例在第 7 天后明显升高。此外，感染组还观察到急性和慢性组织学表现。该模型有助于阐明急性和慢性骨髓炎的病理生理学，并评估新型抗生素或抗菌植入物的效果。
