Department of Clinical Medicine, University of Bologna, 40138 Bologna, Italy.
World J Gastroenterol. 2011 Oct 28;17(40):4496-502. doi: 10.3748/wjg.v17.i40.4496.
To elucidate cell proliferation in erosive reflux disease (ERD) and non-erosive reflux disease (NERD), we evaluated markers in squamous epithelial cells.
Thirty-four consecutive patients with gastroesophageal-reflux-disease-related symptoms (21 NERD and 13 ERD) were evaluated for the enrolment into the study. All patients underwent 24-h pH monitoring, standard endoscopy, and biopsy for histological evaluation. The expression of cyclins D and A was evaluated by real-time reverse transcription polymerase chain reaction (RT-PCR) from isolated epithelial cells. In all samples, analysis of the isolated cell population revealed the presence of epithelial cells only.
Real-time RT-PCR showed that, in patients with ERD, the relative expression of cyclin D1 mRNA in esophageal epithelium was strongly decreased in comparison with NERD patients. The mean value of relative expression of cyclin D1 mRNA in NERD patients was 3.44 ± 1.9, whereas in ERD patients, it was 1.32 ± 0.87 (P = 0.011). Real-time RT-PCR showed that, in patients with ERD, relative expression of cyclin A mRNA in esophageal epithelium was decreased in comparison with that in NERD patients (2.31 ± 2.87 vs 0.66 ± 1.11). The mean bromodeoxyuridine labeling index in the NERD patients was 5.42% ± 1.68%, whereas in ERD patients, it was 4.3% ± 1.59%.
We confirmed reduced epithelial proliferation in ERD compared with NERD patients, and that individuals who develop ERD are characterized by weaker epithelial cell proliferation.
为了阐明糜烂性反流病（ERD）和非糜烂性反流病（NERD）中的细胞增殖情况，我们评估了鳞状上皮细胞中的标志物。
连续评估了 34 例具有胃食管反流病相关症状的患者（21 例 NERD 和 13 例 ERD），以将其纳入研究。所有患者均接受了 24 小时 pH 监测、标准内镜检查和活检以进行组织学评估。通过实时逆转录聚合酶链反应（RT-PCR）从分离的上皮细胞中评估细胞周期蛋白 D 和 A 的表达。在所有样本中，对分离的细胞群体进行分析显示仅存在上皮细胞。
实时 RT-PCR 显示，与 NERD 患者相比，ERD 患者食管上皮中 cyclin D1 mRNA 的相对表达明显降低。NERD 患者 cyclin D1 mRNA 的相对表达平均值为 3.44 ± 1.9，而 ERD 患者为 1.32 ± 0.87（P = 0.011）。实时 RT-PCR 显示，与 NERD 患者相比，ERD 患者食管上皮中 cyclin A mRNA 的相对表达降低（2.31 ± 2.87 对 0.66 ± 1.11）。NERD 患者的溴脱氧尿苷标记指数平均值为 5.42% ± 1.68%，而 ERD 患者为 4.3% ± 1.59%。
我们证实 ERD 患者的上皮增殖减少，并且发生 ERD 的个体的上皮细胞增殖较弱。
