J Biomed Opt. 2011 Nov;16(11):110504. doi: 10.1117/1.3655354.
We have used an adaptive optics confocal scanning laser ophthalmoscope to assess oxygen saturation in small retinal vessels. Images of the vessels with a diameter smaller than 50 μm are recorded at oxygen sensitive and isosbestic wavelengths (680 and 796 nm, respectively). The vessel optical densities (ODs) are determined by a computer algorithm. Then, OD ratios (ODRs), which are inversely proportional to oxygen saturation, are calculated. The results show that arterial ODRs are significantly smaller than venous ODRs, indicating that oxygen saturation in the artery is higher than that in the vein. To the best of our knowledge, this is the first noninvasive measurement of oxygen saturation in small retinal vessels.
我们使用自适应光学共焦扫描激光检眼镜来评估小视网膜血管中的氧饱和度。记录直径小于 50μm 的血管在氧敏感和等消色波长（分别为 680nm 和 796nm）下的图像。血管光密度（OD）由计算机算法确定。然后，计算出与氧饱和度成反比的 OD 比值（ODR）。结果表明，动脉 ODR 明显小于静脉 ODR，表明动脉中的氧饱和度高于静脉。据我们所知，这是首次对小视网膜血管中的氧饱和度进行非侵入性测量。
