秘鲁皮肤利什曼病患者临床样本中利什曼原虫（Viannia）种属鉴定：分子逐步分析方法评估。
Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: assessment of a molecular stepwise approach.
机构信息
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.
出版信息
J Clin Microbiol. 2012 Feb;50(2):495-8. doi: 10.1128/JCM.05061-11. Epub 2011 Nov 23.
Abstract
We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.
摘要
我们提出了一种基于三个 PCR 检测的算法，用于鉴定利什曼原虫（Viannia）种属，并使用来自秘鲁患者的 70 个样本评估了其性能。检测靶标的顺序可以根据物种的流行程度进行排列。按照算法的顺序进行检测，每一步都可以减少大约 30%的样本数量。
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