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秘鲁皮肤利什曼病患者临床样本中利什曼原虫（Viannia）种属鉴定：分子逐步分析方法评估。

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: assessment of a molecular stepwise approach.

机构信息

Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru.

出版信息

J Clin Microbiol. 2012 Feb;50(2):495-8. doi: 10.1128/JCM.05061-11. Epub 2011 Nov 23.

DOI:10.1128/JCM.05061-11
PMID:22116151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3264178/
Abstract

We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

摘要

我们提出了一种基于三个 PCR 检测的算法，用于鉴定利什曼原虫（Viannia）种属，并使用来自秘鲁患者的 70 个样本评估了其性能。检测靶标的顺序可以根据物种的流行程度进行排列。按照算法的顺序进行检测，每一步都可以减少大约 30%的样本数量。

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J Clin Microbiol. 2011 Mar;49(3):1097-100. doi: 10.1128/JCM.02457-10. Epub 2010 Dec 22.
2
Evaluation and improvement of two PCR targets in molecular typing of clinical samples of Leishmania patients.评价和改进两种 PCR 靶标在利什曼病患者临床样本分子分型中的应用。
Exp Parasitol. 2011 Jan;127(1):36-41. doi: 10.1016/j.exppara.2010.06.024. Epub 2010 Jun 22.
3
Detection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis.从美国皮肤利什曼病患者滤纸皮损印迹中检测和鉴定利什曼原虫 DNA。
Clin Infect Dis. 2010 Jan 1;50(1):e1-6. doi: 10.1086/648730.
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Phylogeny of Leishmania species based on the heat-shock protein 70 gene.基于热休克蛋白 70 基因的利什曼原虫种系发生。
Infect Genet Evol. 2010 Mar;10(2):238-45. doi: 10.1016/j.meegid.2009.11.007. Epub 2009 Nov 11.
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Development of a multilocus microsatellite typing approach for discriminating strains of Leishmania (Viannia) species.一种用于区分利什曼原虫（维扬尼亚种）菌株的多位点微卫星分型方法的开发。
J Clin Microbiol. 2009 Sep;47(9):2818-25. doi: 10.1128/JCM.00645-09. Epub 2009 Jul 8.
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Clinical and parasite species risk factors for pentavalent antimonial treatment failure in cutaneous leishmaniasis in Peru.秘鲁皮肤利什曼病中五价锑治疗失败的临床和寄生虫种类风险因素
Clin Infect Dis. 2008 Jan 15;46(2):223-31. doi: 10.1086/524042.
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Influence of Leishmania (Viannia) species on the response to antimonial treatment in patients with American tegumentary leishmaniasis.利什曼原虫（维安亚属）种类对美洲皮肤利什曼病患者抗锑治疗反应的影响。
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Multiple hybrid genotypes of Leishmania (viannia) in a focus of mucocutaneous Leishmaniasis.黏膜皮肤利什曼病疫源地中多种利什曼原虫（维扬亚利什曼原虫）杂交基因型
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