School of Pharmacy, Chouksey Engineering College, Bilaspur, India.
Asian Pac J Trop Med. 2011 Dec;4(12):978-84. doi: 10.1016/S1995-7645(11)60230-7.
To assess the effect of plumbagin-free alcohol extract (PFAE) of Plumbago zeylanica Linn. (Plumbaginaceae) (P. zeylanica) root, on female reproductive system and fertility of adult female wistar rats.
After the oral acute toxicity study, the PFAE was administered at two dose levels to perform the estrous cycle study, anti-implantation and abortifacient activity and hormonal analysis. However, the estrogenic/antiestrogenic activity was evaluated at only one most effective dose.
LD(50) cut-off was 5,000 mg/kg body weight. The extract exhibited significant anti-implantation and abortifacient activity at the tested dose levels (300 and 500 mg/kg, p.o.) (P<0.01). The extract dose-dependently decreased the levels of serum progesterone, follicle stimulating hormone and luteinizing hormone, while a dose-dependent increase was observed in the concentration of serum prolactin. The extract did not show any significant changes in structure and function of uterus when given alone, but when given along with ethinyl estradiol, it exhibited significant antiestrogenic activity in immature overiectomized female rats(P<0.001). Biochemical parameters in the serum/blood and haematological parameters did not show appreciable changes throughout and after the course of investigation. However, all the altered parameters returned to normalcy within 30 days following withdrawal of treatment.
All findings suggest that the antifertility activity of extract could possibly be through the changes in the implantation site, altered hormonal levels, prolonged estrous cycle and anti-estrogenic activity. Hence, the extract possesses reversible antifertility activity without adverse toxicity in female rats.
评估白花丹素-free 醇提物（PFAE）对白花丹根（白花丹科）（P.zeylanica）对成年雌性 Wistar 大鼠生殖系统和生育能力的影响。
在进行急性口服毒性研究后，将 PFAE 以两个剂量水平给药，以进行发情周期研究、抗着床和堕胎活性以及激素分析。然而，仅在一个最有效剂量下评估了雌激素/抗雌激素活性。
LD（50）截止值为 5000mg/kg 体重。在测试剂量水平（300 和 500mg/kg，po）下，提取物表现出显著的抗着床和堕胎活性（P<0.01）。提取物剂量依赖性地降低血清孕激素、卵泡刺激素和黄体生成素水平，而血清催乳素浓度则呈剂量依赖性增加。单独给予提取物时，对子宫的结构和功能没有显示出任何显著变化，但与乙炔雌二醇一起给予时，它在未成熟去卵巢雌性大鼠中表现出显著的抗雌激素活性（P<0.001）。血清/血液中的生化参数和血液学参数在整个研究过程中和研究结束后均未显示出明显变化。然而，所有改变的参数在停药后 30 天内恢复正常。
所有发现表明，提取物的抗生育活性可能是通过改变着床部位、改变激素水平、延长发情周期和抗雌激素活性来实现的。因此，该提取物在雌性大鼠中具有可逆的抗生育活性，且无不良毒性。
