Alhaj Wahibah Taher, Moharram Bushra Abdulkarim, Al-Maqtari Tareq, Al-Mahbashi Hassan M, Al-Doaiss Amin A
Department of Pharmacognosy, Faculty of Pharmacy, Sana'a University, Sana'a, Yemen.
Department of Pharmacology, Faculty of Pharmacy, Sana'a University, Sana'a, Yemen.
Evid Based Complement Alternat Med. 2022 Feb 21;2022:1365526. doi: 10.1155/2022/1365526. eCollection 2022.
(family, Euphorbiaceae) is native to Yemen, where it is commonly known as the Ebki shrub. The fruits of the plant are traditionally ingested by local women as a natural method of contraception. This study was undertaken to investigate the phytochemical content of the methanol extract of fruits and to evaluate its antifertility potential.
Isolation of the chemical constituents was performed by chromatographic techniques, and the chemical structures of these compounds were identified by spectroscopy. The antifertility activity of the methanol extract was assessed in two experimental rat models to explore both the anti-implantation and the estrogenic/antiestrogenic activities in females. In these models, the number of successful implants, the size of litter, and body/ovary weights were all recorded. The development of ovarian follicles was also monitored histological staining.
Phytochemical work on the fruit extract of led to the isolation of two oils (JF1 and JF2) and methyl elaidate. GC-MS analysis of the JF1 oil revealed that the major chemical constituents were fatty acid esters (43.77%), hydrocarbon alkanes (20.65%), and terpenoids (4.65%), while terpenoids (28.8%), fatty acids and their esters, (29.47%), and phytosterol (10.49%) were the major components found in the JF2 oil. The methanol extract of fruit exhibited 50% and 93% abortifacient activity at 150 and 300 mg/kg doses, respectively. The extract also showed significant estrogenic activity as evidenced by the increase in rat ovary weight at a dose of 300 mg/kg compared to the control group. Histological analyses further confirmed this estrogenic activity.
fruits possess an antifertility activity that appeared to result from its antiembryo implantation potential and from its estrogenic activity. The bioactive constituents involved in these activities may need to be further explored and exploited in the pursuit of newer contraceptives.
（大戟科）原产于也门，当地俗称埃布基灌木。当地女性传统上会食用该植物的果实作为一种天然避孕方法。本研究旨在调查该植物果实甲醇提取物的植物化学成分，并评估其抗生育潜力。
通过色谱技术分离化学成分，并通过光谱鉴定这些化合物的化学结构。在两种实验大鼠模型中评估甲醇提取物的抗生育活性，以探究其对雌性大鼠的抗着床以及雌激素/抗雌激素活性。在这些模型中，记录成功着床的数量、窝仔大小以及体重/卵巢重量。还通过组织学染色监测卵巢卵泡的发育情况。
对该植物果实提取物进行植物化学研究，分离出两种油（JF1和JF2）以及反油酸甲酯。对JF1油的气相色谱 - 质谱分析表明，主要化学成分是脂肪酸酯（43.77%）、烃类烷烃（20.65%）和萜类化合物（4.65%），而JF2油中的主要成分是萜类化合物（28.8%）、脂肪酸及其酯（29.47%）和植物甾醇（10.49%）。该植物果实的甲醇提取物在150和300mg/kg剂量下分别表现出50%和93%的堕胎活性。与对照组相比，该提取物在300mg/kg剂量下使大鼠卵巢重量增加，表明其具有显著的雌激素活性。组织学分析进一步证实了这种雌激素活性。
该植物果实具有抗生育活性，这似乎源于其抗胚胎着床潜力和雌激素活性。在开发新型避孕药的过程中，可能需要进一步探索和利用参与这些活性的生物活性成分。
