Al-Fahdawi Mohamed Qasim, Aldoghachi Ahmed Faris, Alhassan Fatah H, Al-Doghachi Faris A J, Alshwyeh Hussah Abdullah, Rasedee Abdullah, Alnasser Sulaiman Mohammed, Al-Qubaisi Mothanna Sadiq, Ibrahim Wisam Nabeel
Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Faculty of Medicine and Health Sciences, University Putra Malaysia, UPM, Serdang, 43300, Malaysia.
Heliyon. 2023 Apr 17;9(5):e15560. doi: 10.1016/j.heliyon.2023.e15560. eCollection 2023 May.
Silver-doped magnesia nanoparticles (Ag/MgO) were synthesized using the precipitation method and characterized by various techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA), Brunner-Emmett-Teller (BET) surface area measurements, and dispersive X-ray spectroscopy (EDX). The morphology of Ag/MgO nanoparticles was determined by transmission and scanning electron microscopy, which revealed cuboidal shaped nanoparticles with sizes ranging from 31 to 68 nm and an average size of 43.5 ± 10.6 nm. The anticancer effects of Ag/MgO nanoparticles were evaluated on human colorectal (HT29) and lung adenocarcinoma (A549) cell lines, and their caspase-3, -8, and -9 activities, as well as Bcl-2, Bax, p53, cytochrome C protein expressions were estimated. Ag/MgO nanoparticles showed selective toxicity towards HT29 and A549 cells while remaining relatively innocuous towards the normal human colorectal, CCD-18Co, and lung, MRC-5 cells. The IC values of Ag/MgO nanoparticles on the HT29 and A549 cells were found to be 90.2 ± 2.6 and 85.0 ± 3.5 μg/mL, respectively. The Ag/MgO nanoparticles upregulated caspase-3 and -9 activities, downregulated Bcl-2, upregulated Bax and p53 protein expressions in the cancer cells. The morphology of the Ag/MgO nanoparticle treated HT29 and A549 cells was typical of apoptosis, with cell detachment, shrinkage, and membrane blebbing. The results suggest that Ag/MgO nanoparticles induce apoptosis in cancer cells and exhibit potential as a promising anticancer agent.
采用沉淀法合成了银掺杂氧化镁纳米颗粒（Ag/MgO），并通过多种技术对其进行了表征，如X射线衍射（XRD）、傅里叶变换红外光谱（FT-IR）、热重分析（TGA）、布鲁诺-埃米特-泰勒（BET）表面积测量以及能量色散X射线光谱（EDX）。通过透射电子显微镜和扫描电子显微镜确定了Ag/MgO纳米颗粒的形态，结果显示其为立方体形纳米颗粒，尺寸范围为31至68纳米，平均尺寸为43.5±10.6纳米。评估了Ag/MgO纳米颗粒对人结肠直肠癌（HT29）和肺腺癌（A549）细胞系的抗癌作用，并估计了它们的半胱天冬酶-3、-8和-9活性以及Bcl-2、Bax、p53、细胞色素C蛋白表达。Ag/MgO纳米颗粒对HT29和A549细胞表现出选择性毒性，而对正常人结肠直肠CCD-18Co细胞和肺MRC-5细胞相对无害。发现Ag/MgO纳米颗粒对HT29和A549细胞的IC值分别为90.2±2.6和85.0±3.μg/mL。Ag/MgO纳米颗粒上调了癌细胞中的半胱天冬酶-3和-9活性，下调了Bcl-2，上调了Bax和p53蛋白表达。经Ag/MgO纳米颗粒处理的HT29和A549细胞的形态具有典型的凋亡特征，包括细胞脱离、收缩和膜泡形成。结果表明，Ag/MgO纳米颗粒可诱导癌细胞凋亡，并具有作为有前景的抗癌剂的潜力。
