Department of Genetics, Comenius University, Bratislava, Slovakia.
Cell Biochem Funct. 2012 Mar;30(2):101-7. doi: 10.1002/cbf.1822. Epub 2011 Nov 28.
The objectives of this study were to examine whether the methanolic and aqueous extracts from the haulm and flower of Gentiana asclepiadea exhibited free radical scavenging and protective (antigenotoxic) effect against DNA oxidation induced by H(2)O(2) in human lymphocytes and human embryonic kidney cells (HEK 293). All four extracts exhibited high scavenging effect on 1,1-diphenyl-2-picrylhydrazyl radicals at concentrations 2.5 and 25 mg ml(-1). The level of DNA damage was measured using the alkaline version of single-cell gel electrophoresis (comet assay). Challenge with H(2)O(2) shows that the pre-treatment of the cells with non-genotoxic doses of Gentiana extracts protected human DNA-either eliminated or significantly reduced H(2)O(2) induced DNA damage. The genotoxic activity of H(2)O(2) was most effectively decreased after 30 min of pre-incubation with 0.05 mg ml(-1) (range, 93.5%-96.3% of reduction in lymphocytes) and 0.25 mg ml(-1) (range, 59.5%-71.4% and 52.7%-66.4% of reduction in lymphocytes and HEK 293 cells, respectively) of G. asclepiadea extracts. These results suggest that the tested G. asclepiadea extracts could be considered as an effective natural antioxidant source.
本研究旨在考察獐牙菜茎叶和花的甲醇和水提取物是否具有清除自由基和保护（抗原毒性）作用，以防止 H(2)O(2)诱导的人淋巴细胞和人胚肾细胞（HEK 293）DNA 氧化。四种提取物在 2.5 和 25mg/ml 浓度下对 1,1-二苯基-2-苦基肼自由基均表现出高清除作用。使用碱性单细胞凝胶电泳（彗星试验）测定 DNA 损伤水平。用 H(2)O(2)进行攻击表明，用非遗传毒性剂量的獐牙菜提取物预处理细胞可保护人 DNA-消除或显著降低 H(2)O(2)诱导的 DNA 损伤。用 0.05mg/ml（淋巴细胞中减少 93.5%-96.3%）和 0.25mg/ml（淋巴细胞中减少 59.5%-71.4%，HEK 293 细胞中减少 52.7%-66.4%）的獐牙菜提取物进行 30 分钟预孵育后，H(2)O(2)的遗传毒性活性降低最为明显。这些结果表明，测试的獐牙菜提取物可以被认为是一种有效的天然抗氧化剂来源。
