Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina, Bratislava 842 15, Slovakia.
Food Chem Toxicol. 2012 Sep;50(9):3352-9. doi: 10.1016/j.fct.2012.06.017. Epub 2012 Jun 18.
Exposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column derivatisation, and their potential ability to enhance DNA repair in human lymphocytes after hydrogen peroxide (H(2)O(2)) treatment (250 μM, 5 min). We also studied DNA repair in human kidney HEK 293 cells after exposure to 20 nm silver nanoparticles (AgNPs) (100 μg/ml, 30 min) in the presence and absence of the plant extract. We have found that mangiferin along with unidentified polar compounds are the most pronounced antioxidants in the studied extracts. Extract from haulm exhibited slightly stronger antioxidant properties compared to flower extracts. However, all four extracts showed significant ability to enhance DNA repair in both cell types after H(2)O(2) and AgNP treatments.
已知暴露于高水平的不同环境污染物与人类 DNA 损伤的诱导有关。因此，DNA 修复对于预防突变非常重要，对预防癌症至关重要。在我们的研究中，我们专注于定量分析龙胆属植物水或甲醇提取物，这些提取物来自花和茎，它们在 ABTS 柱后衍生化中的抗氧化能力，以及它们在过氧化氢 (H2O2) 处理后（250 μM，5 分钟）增强人淋巴细胞 DNA 修复的潜在能力。我们还研究了在存在和不存在植物提取物的情况下，人类肾 HEK 293 细胞暴露于 20nm 银纳米颗粒 (AgNPs)（100μg/ml，30 分钟）后 DNA 修复情况。我们发现，芒果苷和未鉴定的极性化合物是研究提取物中最显著的抗氧化剂。与花提取物相比，茎提取物表现出稍强的抗氧化特性。然而，所有四种提取物在 H2O2 和 AgNP 处理后均显示出在两种细胞类型中增强 DNA 修复的显著能力。
