Pariset Lorraine, Mariotti Marco, Gargani Maria, Joost Stephane, Negrini Riccardo, Perez Trinidad, Bruford Michael, Ajmone Marsan Paolo, Valentini Alessio
Department for Innovation in Biological, Agro-Food and Forest Systems, Tuscia University, 01100 Viterbo, Italy.
ScientificWorldJournal. 2011;11:1641-59. doi: 10.1100/2011/186342. Epub 2011 Sep 13.
We employed mtDNA and nuclear SNPs to investigate the genetic diversity of sheep breeds of three countries of the Mediterranean basin: Albania, Greece, and Italy. In total, 154 unique mtDNA haplotypes were detected by means of D-loop sequence analysis. The major nucleotide diversity was observed in Albania. We identified haplogroups, A, B, and C in Albanian and Greek samples, while Italian individuals clustered in groups A and B. In general, the data show a pattern reflecting old migrations that occurred in postneolithic and historical times. PCA analysis on SNP data differentiated breeds with good correspondence to geographical locations. This could reflect geographical isolation, selection operated by local sheep farmers, and different flock management and breed admixture that occurred in the last centuries.
我们利用线粒体DNA(mtDNA)和核单核苷酸多态性(SNP)来研究地中海盆地三个国家(阿尔巴尼亚、希腊和意大利)绵羊品种的遗传多样性。通过D-loop序列分析,共检测到154种独特的mtDNA单倍型。阿尔巴尼亚观察到主要的核苷酸多样性。我们在阿尔巴尼亚和希腊的样本中鉴定出单倍群A、B和C,而意大利个体则聚类为A组和B组。总体而言,数据显示出一种反映新石器时代后期和历史时期发生的古老迁徙的模式。对SNP数据的主成分分析(PCA)区分了品种,与地理位置有很好的对应关系。这可能反映了地理隔离、当地绵羊养殖户进行的选择,以及过去几个世纪发生的不同的畜群管理和品种混合。