Department of Animal Breeding, Nutrition and Laboratory Animal Science, University of Veterinary Medicine, Budapest, Hungary.
Federal Ministry of Agriculture, Forestry, Environment and Water Management, Institute for Organic Agriculture and Biodiversity, Wels-Thalheim, Austria.
Vet Med Sci. 2021 Nov;7(6):2330-2338. doi: 10.1002/vms3.585. Epub 2021 Jul 22.
The consideration of the descendance is indispensable in the preservation of endangered animal breeds. The authors compared mitochondrial DNA (mtDNA) control region sequence in three descendant breeds of the extinct Zaupel sheep, firstly. Their investigation was carried out in order to prove the common origin of Waldschaf (Austria), Bovec sheep (Slovenia) and Cikta (Hungary). A total of 118 biological samples were taken from non-related representatives of the three breeds between 2015 and 2017. A newly designed primer pair was also used to amplify the segment (1180 bp) to be tested. The total number of haplotypes in the whole study population was 49. The majority of which fell into haplogroup B. The significant negative value of the Fu's Fs statistic (Fs statistic = -3.296, p = 0.013) based on haplotype frequencies demonstrated a moderate foreign gene flow. As a novel observation haplogroups C and D appeared in Cikta and Bovec sheep, respectively. The Tajima D-test value in the entire study population was -0.914 (p > 0.10), meaning that the separation of the three descendant breeds did not cause genetic drift, these are collectively in genetic equilibrium. The genetic information confirmed the common origin of the breeds known from the breed history.
在保护濒危动物品种时，必须考虑其后代。作者首先比较了灭绝的扎普尔羊的三个后裔品种的线粒体 DNA（mtDNA）控制区序列。他们的调查旨在证明 Waldschaf（奥地利）、Bovec 绵羊（斯洛文尼亚）和 Cikta（匈牙利）这三个品种的共同起源。2015 年至 2017 年期间，从这三个品种的非相关代表中采集了 118 个生物样本。还使用了一对新设计的引物来扩增要测试的片段（1180bp）。整个研究群体的单倍型总数为 49 个。其中大多数属于单倍型 B。基于单倍型频率的 Fu's Fs 统计量的显著负值（Fs 统计量=-3.296，p=0.013）表明存在中等程度的外源基因流。作为一个新的观察结果，单倍型 C 和 D 分别出现在 Cikta 和 Bovec 绵羊中。整个研究群体的 Tajima D 检验值为-0.914（p>0.10），这意味着三个后裔品种的分离没有引起遗传漂变，它们共同处于遗传平衡状态。遗传信息证实了这些品种从品种历史上已知的共同起源。
