Department of Maxillofacial and Plastic Surgery, South Western Sydney Medical School, University of Sydney, Sydney, Australia.
Wound Repair Regen. 2012 Jan-Feb;20(1):83-90. doi: 10.1111/j.1524-475X.2011.00747.x. Epub 2011 Nov 29.
Chronic non-healing wounds affect a significant number of patients worldwide. Although the etiologies of these wounds are varied, bacterial infection has been suggested as a major factor responsible for the perpetual inflammation and tissue destruction observed in such wounds. Recent evidence has emerged suggesting that bacterial biofilms in particular may have a significant role in this process. At the same time, topical negative pressure dressing is gaining acceptance as a therapy which promotes healing in recalcitrant wounds. In this study an in vitro Pseudomonas aeruginosa biofilm model was developed to mimic potential surface wound biofilms. Topical negative pressure dressing was applied to the model and the effects of topical negative pressure dressing on the in vitro wound biofilms were examined using both quantitative microbiological counting technique and imaging studies. The results demonstrated a small but statistically significant reduction in biofilm bacteria at 2 weeks when exposed to topical negative pressure. When this was combined with silver impregnated foam, the reduction was far more significant and was observable within 24 hours. Microscopically, it was also noted that topical negative pressure compressed the biofilm architecture with a reduction in thickness and diffusion distance.
慢性难愈性创面影响着全球大量的患者。尽管这些创面的病因多种多样，但细菌感染已被认为是导致此类创面持续炎症和组织破坏的主要因素。最近的证据表明，细菌生物膜可能在这一过程中起着重要作用。与此同时，负压伤口治疗技术作为一种促进难治性创面愈合的治疗方法正被广泛接受。在这项研究中，建立了体外铜绿假单胞菌生物膜模型来模拟潜在的表面创面生物膜。将负压伤口治疗敷料应用于模型，通过定量微生物计数技术和成像研究来检测负压伤口治疗敷料对体外创面生物膜的影响。结果表明，在暴露于负压下的第 2 周，生物膜细菌数量略有但具有统计学意义的减少。当与载银泡沫敷料联合使用时，减少量更为显著，在 24 小时内即可观察到。显微镜下还观察到，负压压缩了生物膜结构，使其厚度和扩散距离减小。
