Muro Y, Sugimoto K, Okazaki T, Ohashi M
Department of Dermatology, Nagoya University School of Medicine, Aichi, Japan.
J Rheumatol. 1990 Aug;17(8):1042-7.
We tested anticentromere antibody positive sera from 37 patients by immunoblotting techniques. Three antigenic polypeptides were recognized when immunoblotted against protein extracts from HeLa cell nuclei or from chromosomal segments enriched with centromere region. These were a 17 kDa (CENP-A recognized by 34 sera), an 80 kDa (CENP-B recognized by 33 sera), and a 140 kDa polypeptide (CENP-C recognized by 26 sera). There was no disease specific pattern of antigenic polypeptides, although Raynaud's phenomenon was frequent in patients with anti-CENP-B reactivities (p less than 0.01). The heterogeneity of the anticentromere antibody response in Japanese patients shows anticentromere antibody may not be a disease specific autoantibody and diagnostic marker.
我们采用免疫印迹技术检测了37例抗着丝粒抗体阳性患者的血清。当用来自HeLa细胞核或富含着丝粒区域的染色体片段的蛋白质提取物进行免疫印迹时，识别出三种抗原多肽。它们分别是17 kDa（34份血清识别出的CENP-A）、80 kDa（33份血清识别出的CENP-B）和140 kDa多肽（26份血清识别出的CENP-C）。尽管抗CENP-B反应性的患者中雷诺现象很常见（p小于0.01），但抗原多肽并没有疾病特异性模式。日本患者抗着丝粒抗体反应的异质性表明，抗着丝粒抗体可能不是疾病特异性自身抗体和诊断标志物。
