HIV前病毒两侧的宿主序列。
Host sequences flanking the HIV provirus.
作者信息
Vincent K A, York-Higgins D, Quiroga M, Brown P O
机构信息
Howard Hughes Medical Institute, Stanford University School of Medicine, CA 94305.
出版信息
Nucleic Acids Res. 1990 Oct 25;18(20):6045-7. doi: 10.1093/nar/18.20.6045.
Abstract
A conserved property of retroviral proviruses is the presence of a direct repeat in the host DNA immediately flanking the viral sequence; each virus generates a repeat with a characteristic length. By sequencing the viral/host DNA junctions from five HIV-1 proviral clones, we have confirmed that integration of HIV results in the generation of a five basepair direct repeat. A target sequence in uninfected host DNA was analyzed to establish that the five basepair sequence flanking the provirus was present only once prior to integration. Of the five proviruses examined, two were found to have integrated in known repetitive sequence elements of the human genome; one in a Line-1 element and a second in satellite DNA.
摘要
逆转录病毒前病毒的一个保守特性是，在紧邻病毒序列的宿主DNA中存在一个直接重复序列；每种病毒都会产生一个具有特征长度的重复序列。通过对五个HIV-1前病毒克隆的病毒/宿主DNA连接处进行测序，我们证实HIV的整合会导致产生一个五碱基对的直接重复序列。对未感染宿主DNA中的一个靶序列进行分析后确定，前病毒两侧的五碱基对序列在整合之前只出现过一次。在所检测的五个前病毒中，发现有两个整合到了人类基因组已知的重复序列元件中；一个整合到了LINE-1元件中，另一个整合到了卫星DNA中。
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本文引用的文献
1
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95. doi: 10.1093/nar/12.1part1.387.
2
Human satellite I sequences include a male specific 2.47 kb tandemly repeated unit containing one Alu family member per repeat.人类卫星I序列包括一个男性特异性的2.47 kb串联重复单元，每个重复单元包含一个Alu家族成员。
Nucleic Acids Res. 1984 Mar 26;12(6):2887-900. doi: 10.1093/nar/12.6.2887.
3
A Rous sarcoma virus provirus is flanked by short direct repeats of a cellular DNA sequence present in only one copy prior to integration.劳氏肉瘤病毒前病毒两侧是整合前仅以单拷贝形式存在的细胞DNA序列的短正向重复序列。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4299-303. doi: 10.1073/pnas.78.7.4299.
4
No apparent nucleotide sequence specificity in cellular DNA juxtaposed to retrovirus proviruses.与逆转录病毒前病毒并列的细胞DNA中无明显的核苷酸序列特异性。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7357-61. doi: 10.1073/pnas.77.12.7357.
5
Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.整合的莫洛尼肉瘤前病毒长末端重复序列及其宿主与病毒连接区的核苷酸序列。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937-41. doi: 10.1073/pnas.77.7.3937.
6
Molecular cloning of AIDS-associated retrovirus.艾滋病相关逆转录病毒的分子克隆
Nature. 1984;312(5996):760-3. doi: 10.1038/312760a0.
7
Molecular characterization of human T-cell leukemia (lymphotropic) virus type III in the acquired immune deficiency syndrome.获得性免疫缺陷综合征中人类T细胞白血病（嗜淋巴细胞）病毒III型的分子特征
Science. 1984 Dec 7;226(4679):1165-71. doi: 10.1126/science.6095449.
8
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9
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