Ryu So-Young, Hong Jin-Sung, Rhee Sun-Ju, Lee Gung Pyo
Department of Integrative Plant Science, Chung-Ang University, Ansung 456-756, Korea.
Virus Genes. 2012 Apr;44(2):345-8. doi: 10.1007/s11262-011-0694-5. Epub 2011 Dec 6.
Ribgrass mosaic virus (RMV) has severely decreased the production and lowered quality of Chinese cabbage co-infected with Turnip mosaic virus (63.4%) in Korea. The complete genome sequence of RMV isolated from Brassica rapa ssp. pekinensis was determined. The full genome consisted of 6,304 nucleotides and showed sequence identities of 91.5-94.2% with the corresponding genome of other RMV strains. Full-length cDNA of RMV-Br was amplified by RT-PCR with a 5'-end primer harboring a T7 promoter sequence and a 3'-end RMV specific primer. Subsequently, the full-length cDNA was cloned into plasmid vectors. Capped transcripts synthesized from the cDNA clone were highly infectious and caused characteristic symptoms in B. rapa ssp. pekinensis and several indicator plants, similar to wild type RMV. Since there has not been found RMV resistant Chinese cabbage yet and the virus has been prevalent already throughout the natural fields of Korea, the identification of full sequence and development of infectious clone would help developing breeding program for RMV resistant crops.
苎麻花叶病毒(RMV)已严重降低了韩国与芜菁花叶病毒共同感染的大白菜的产量,并降低了其品质(感染率为63.4%)。测定了从白菜型油菜中分离出的RMV的全基因组序列。该全基因组由6304个核苷酸组成,与其他RMV株系的相应基因组的序列同一性为91.5%-94.2%。利用含有T7启动子序列的5'端引物和3'端RMV特异性引物,通过RT-PCR扩增RMV-Br的全长cDNA。随后,将全长cDNA克隆到质粒载体中。从cDNA克隆合成的加帽转录本具有高度传染性,并在白菜型油菜和几种指示植物中引起特征性症状,类似于野生型RMV。由于尚未发现抗RMV的大白菜,且该病毒已在韩国的自然田间普遍存在,因此全序列的鉴定和感染性克隆的开发将有助于制定抗RMV作物的育种计划。