State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Chem Commun (Camb). 2012 Jan 25;48(7):997-9. doi: 10.1039/c1cc15407a. Epub 2011 Dec 5.
A new label-free method for the detection of apoptosis was proposed based on colorimetric assay of caspase-3 activity using an unlabeled Asp-Glu-Val-Asp (DEVD)-containing peptide substrate and unmodified gold nanoparticles (AuNPs).
基于 caspase-3 活性的比色测定,提出了一种新的用于检测细胞凋亡的无标记方法,该方法使用未标记的含有 Asp-Glu-Val-Asp (DEVD) 的肽底物和未经修饰的金纳米粒子(AuNPs)。