Department of NanoEngineering, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), Singapore, 138634, Singapore.
Angew Chem Int Ed Engl. 2023 Jan 23;62(4):e202214394. doi: 10.1002/anie.202214394. Epub 2022 Dec 15.
Aromatic interactions are commonly involved in the assembly of naturally occurring building blocks, and these interactions can be replicated in an artificial setting to produce functional materials. Here we describe a colorimetric biosensor using co-assembly experiments with plasmonic gold and surfactant-like peptides (SLPs) spanning a wide range of aromatic residues, polar stretches, and interfacial affinities. The SLPs programmed in DDD-(ZZ) -FFPC self-assemble into higher-order structures in response to a protease and subsequently modulate the colloidal dispersity of gold leading to a colorimetric readout. Results show the strong aggregation propensity of the FFPC tail without polar DDD head. The SLPs were specific to the target protease, i.e., M , a biomarker for SARS-CoV-2. This system is a simple and visual tool that senses M in phosphate buffer, exhaled breath condensate, and saliva with detection limits of 15.7, 20.8, and 26.1 nM, respectively. These results may have value in designing other protease testing methods.
芳香相互作用通常涉及天然构建块的组装，并且可以在人工环境中复制这些相互作用，以生产功能性材料。在这里，我们描述了一种使用等离子体金和表面活性剂样肽（SLP）的共组装实验的比色生物传感器，该实验涵盖了广泛的芳香族残基、极性伸展和界面亲和力。DDD-（ZZ）-FFPC 编程的 SLP 响应蛋白酶自组装成高级结构，随后调节金的胶体分散性，从而产生比色读出。结果表明，没有极性 DDD 头的 FFPC 尾巴具有很强的聚集倾向。SLP 对目标蛋白酶 M 具有特异性，M 是 SARS-CoV-2 的生物标志物。该系统是一种简单直观的工具，可在磷酸盐缓冲液、呼出冷凝物和唾液中检测 M，检测限分别为 15.7、20.8 和 26.1 nM。这些结果在设计其他蛋白酶检测方法方面可能具有价值。
