Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Collaborative Innovation Center for Respiratory Disease Diagnosis and Treatment & Chinese Medicine Development of Henan Province, Zhengzhou 450046, China.
Molecules. 2018 Sep 7;23(9):2293. doi: 10.3390/molecules23092293.
The aim of this study was to investigate the estrogen-like effects of 2-phenylacetamide (PA), which is the main compound isolated from the seeds of Willd (LA). Results showed that LA and PA could promote the proliferation of MCF-7 cells. The mouse uterine weight test showed that, LA and PA could increase the uterus index of immature female mice, and the levels of luteinizing hormone (LH) and estrogen (E2). LA could increase the expression of ER and ER, while PA could increase the expression of ER, ER and GPR30 in the uterus and MCF-7 cells. In addition, co-incubation of the estrogen receptor blocker with LA or PA abolished the inductive effect of the proliferation. PA has estrogenic activities and was the material basis of LA that played the estrogenic effect. LA and PA might be used for the treatment of perimenopause syndrome in a novel application.
本研究旨在探讨 2-苯乙酰胺（PA）的类雌激素作用，PA 是从 Willd 种子中分离得到的主要化合物（LA）。结果表明，LA 和 PA 可促进 MCF-7 细胞增殖。小鼠子宫重量试验表明，LA 和 PA 可增加未成年雌性小鼠的子宫指数，并提高促黄体生成素（LH）和雌激素（E2）水平。LA 可增加 ER 和 ER 的表达，而 PA 可增加子宫和 MCF-7 细胞中 ER、ER 和 GPR30 的表达。此外，用雌激素受体阻滞剂与 LA 或 PA 共孵育可消除增殖的诱导作用。PA 具有雌激素活性，是发挥雌激素作用的 LA 的物质基础。LA 和 PA 可能在治疗围绝经期综合征方面具有新的应用价值。
