Bio-IT Fusion Technology Research Institute, Pusan National University, Busan, Korea.
J Med Food. 2011 Dec;14(12):1519-26. doi: 10.1089/jmf.2011.1656.
Periodontitis, a chronic inflammatory periodontal disease that develops from gingivitis, is caused by periodontal pathogenic bacteria such as Porphyromonas gingivalis. Recent studies have focused on the antioxidant, anti-human immunodeficiency virus, anticarcinogenic, and anti-inflammatory properties of gomisins. However, the anti-inflammatory activities of gomisin plants through heme oxygenase-1 (HO-1) signals remain poorly defined. We found that gomisins' anti-inflammatory activity occurs via the induction of HO-1 expression. Gomisins G and J inhibit the production of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin-1β, and interleukin-6 and also block nuclear factor-κB activation in Raw264.7 cells stimulated with P. gingivalis lipopolysaccharide. Furthermore, pro-inflammatory cytokine production is inhibited through the induction of HO-1 expression. HO-1 expression is induced by all gomisins, but their anti-inflammatory activity via HO-1 signaling is observed with gomisins G and J, and not A. We found that gomisins G and J extracted from Schisandria chinensis can inhibit the P. gingivalis lipopolysaccharide induced-inflammatory responses in Raw264.7 cells.
牙周炎是一种由牙周病原菌如牙龈卟啉单胞菌引起的慢性炎症性牙周疾病。最近的研究集中在 gomisins 的抗氧化、抗人类免疫缺陷病毒、抗癌和抗炎特性上。然而，gomisin 植物通过血红素加氧酶-1（HO-1）信号的抗炎活性仍未得到明确界定。我们发现 gomisins 的抗炎活性是通过诱导 HO-1 表达来实现的。Gomisin G 和 J 抑制促炎细胞因子肿瘤坏死因子-α、白细胞介素-1β 和白细胞介素-6 的产生，并阻断 Raw264.7 细胞中牙龈卟啉单胞菌脂多糖刺激的核因子-κB 激活。此外，通过诱导 HO-1 表达来抑制促炎细胞因子的产生。所有 gomisins 都能诱导 HO-1 表达，但只有 gomisins G 和 J 能通过 HO-1 信号通路发挥抗炎活性，而 gomisins A 则不能。我们发现从五味子中提取的 gomisins G 和 J 可以抑制 Raw264.7 细胞中牙龈卟啉单胞菌脂多糖诱导的炎症反应。
