Vega J A, Ricci A, Amenta F
Departamento de Morfologia y Biologia Celular, Universidad de Oviedo, Spain.
Mech Ageing Dev. 1990 Jun;54(3):185-96. doi: 10.1016/0047-6374(90)90049-l.
The influence of aging on the sympathetic innervation of the kidney was studied in 3- (considered to be young), 12- (considered to be adult) and 24- (considered to be old) month-old male Sprague-Dawley rats by means of high pressure liquid chromatography with electrochemical detection, catecholamine histofluorescence and acetylcholinesterase (AChE) histochemistry. Body and kidney weights were significantly increased in adult in comparison with young rats. No further increase of either body or kidney weight was appreciated in old rats. Noradrenaline levels were increased by about 48% in adult rats and were decreased in old rats (by approx. 22% vs. young and 60% vs. adult). The density of perivascular noradrenergic fibres was significantly increased in adult rats and decreased in old animals. The percentage of kidney glomeruli supplied by AChE-positive nerve fibres is also remarkably increased in 12-month old rats and decreased in 24-month-old rats. The present data indicate that there is a striking increase in the expression of sympathetic innervation of rat kidney at 12 months of age followed by a significant decrease in the expression of innervation in old age. These changes are discussed in relation to the age-dependent impairment of renal function.
通过高压液相色谱-电化学检测、儿茶酚胺组织荧光法和乙酰胆碱酯酶（AChE）组织化学方法，研究了3个月大（视为年轻）、12个月大（视为成年）和24个月大（视为年老）的雄性Sprague-Dawley大鼠衰老对肾交感神经支配的影响。与年轻大鼠相比，成年大鼠的体重和肾脏重量显著增加。老年大鼠的体重和肾脏重量均未进一步增加。成年大鼠去甲肾上腺素水平增加约48%，老年大鼠去甲肾上腺素水平降低（与年轻大鼠相比约降低22%，与成年大鼠相比降低60%）。成年大鼠血管周围去甲肾上腺素能纤维密度显著增加，老年动物则降低。AChE阳性神经纤维供应的肾肾小球百分比在12个月大的大鼠中也显著增加，在24个月大的大鼠中降低。目前的数据表明，大鼠肾脏交感神经支配的表达在12个月龄时显著增加，随后在老年时神经支配表达显著降低。结合肾功能的年龄依赖性损害对这些变化进行了讨论。
