Taborda Natalia, Zapata-Builes Wildeman, Montoya Carlos, Rugeles María Teresa
Immunovirology Group, University of Antioquia, Medellin, Colombia.
AIDS Res Hum Retroviruses. 2012 Sep;28(9):1059-62. doi: 10.1089/AID.2011.0151. Epub 2012 Mar 21.
The exposure to human immunodeficiency virus type 1 (HIV-1) does not always result in infection. Indeed, there are individuals who have been repeatedly exposed to HIV-1 but do not exhibit clinical or serological evidence of infection; they are known as HIV-exposed seronegative individuals (HESN). To determine if secretory leukocyte protease inhibitor (SLPI), a soluble factor secreted by epithelial cells lining mucosal surfaces that showed anti-HIV activity in vitro, was associated with natural resistance to HIV infection, we measured by real time RT-PCR the expression of SLPI in oral mucosa of a cohort of Colombian HESN, in chronically HIV-1-infected individuals and in healthy controls. The HESN expressed significantly higher levels of SLPI mRNA than healthy controls (p=0.033) and chronically infected subjects (p=0.011). These findings suggest an association between SLPI expression and the natural resistance to HIV-1 infection exhibited by our HESN cohort.
暴露于1型人类免疫缺陷病毒（HIV-1）并不总是导致感染。事实上，有一些人反复暴露于HIV-1，但没有表现出感染的临床或血清学证据；他们被称为HIV暴露血清阴性个体（HESN）。为了确定分泌型白细胞蛋白酶抑制剂（SLPI）——一种由黏膜表面上皮细胞分泌的在体外显示出抗HIV活性的可溶性因子——是否与对HIV感染的天然抵抗力有关，我们通过实时逆转录聚合酶链反应（RT-PCR）测量了一组哥伦比亚HESN、慢性HIV-1感染个体和健康对照者口腔黏膜中SLPI的表达。HESN表达的SLPI mRNA水平显著高于健康对照者（p=0.033）和慢性感染受试者（p=0.011）。这些发现表明SLPI表达与我们的HESN队列所表现出的对HIV-1感染的天然抵抗力之间存在关联。
