The French Agency for Safety of Health Products, AFSSAPS, Laboratories and Control Directorate, 635 rue de Garenne, 34748 Vendargues cedex, France.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2012;29(2):161-71. doi: 10.1080/19440049.2011.638676. Epub 2011 Dec 9.
The presence on the market of illegal products for slimming purposes or the treatment of overweight is a public health issue. These products may contain illicit chemicals in order to improve their effectiveness. Some of these weight-loss compounds are responsible for adverse events, including fatal outcomes. A general strategy for the analysis of any suspect formulation begins with a large screening for the general search of a wide range of compounds. A methodology for the qualitative and quantitative determination of 34 compounds in slimming preparations (such as dietary supplements or medicinal products) was used for the control of slimming formulations from the market, including over the Internet. The fast liquid chromatography system (ultra-high-pressure liquid chromatography) used a gradient of solvent (phosphate buffer and acetonitrile), a C18 endcapped column and a diode array detector. This system allows dual identification based on retention time and UV spectra. The analytical method is simple, fast and selective since 34 weight-loss compounds can be detected in a 15-min run time. Thus, 32 commercial slimming formulations were analysed using this method, allowing the detection and quantification of hazardous active substances: caffeine, clenbuterol, nicotinamide, phenolphthalein, rimonabant, sibutramine, didesmethylsibutramine, synephrine and yohimbine.
市售的用于减肥或治疗超重的非法产品是一个公共卫生问题。这些产品可能含有非法化学物质以提高其效果。其中一些减肥化合物会导致不良事件，包括致命后果。任何可疑配方的分析一般策略是首先进行广泛的筛查，以广泛搜索各种化合物。一种用于控制市场上的减肥制剂（如膳食补充剂或药品）的 34 种化合物的定性和定量测定方法已用于控制通过互联网销售的减肥制剂。该快速液相色谱系统（超高效液相色谱）使用溶剂梯度（磷酸盐缓冲液和乙腈）、C18 端封柱和二极管阵列检测器。该系统允许基于保留时间和紫外光谱进行双重鉴定。该分析方法简单、快速且具有选择性，因为可以在 15 分钟的运行时间内检测到 34 种减肥化合物。因此，使用该方法分析了 32 种商业减肥制剂，可检测并定量危险的活性物质：咖啡因、克仑特罗、烟酰胺、酚酞、利莫那班、西布曲明、二甲苯丁胺、辛弗林和育亨宾。
