Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
Molecules. 2011 Dec 8;16(12):10214-26. doi: 10.3390/molecules161210214.
Nine compounds have been isolated from the ethyl acetate soluble fraction of C. sinensis, namely protocatechuic acid (1), trans-caffeic acid (2), methyl rosmarinate (3), rosmarinic acid (4), kaempferide-3-O-β-D-glucopyranoside (5), kaempferol-3-O-β-D-glucopyranoside (6), quercetin-3-O-β-D-glucopyranoside (7), kaempferide-3-O-α-L-rhamnopyranosyl (1→6)-β-D-glucopyranoside (8) and kaempferol-3-O-α-L-rhamno-pyranosyl (1→6)-β-D-glucopyranoside (9), all reported for the first time from this species. The structures of these compounds were deduced on the basis of spectroscopic studies, including 1D and 2D NMR techniques. Compounds 1-9 were investigated for biological activity and showed significant anti-inflammatory activity in the carrageen induced rat paw edema test. The antioxidant activities of isolated compounds 1-9 were evaluated by the DPPH radical scavenging test, and compounds 1, 2, 4 and 7-9 exhibited marked scavenging activity compared to the standard BHA. These compounds were further studied for their anti-glycation properties and some compounds showed significant anti-glycation inhibitory activity. The purity of compounds 2-5, 8 and 9 was confirmed by HPLC. The implications of these results for the chemotaxonomic studies of the genus Cordia have also been discussed.
从黄槐中分离得到 9 种化合物，分别为原儿茶酸（1）、反式咖啡酸（2）、迷迭香酸甲酯（3）、迷迭香酸（4）、山奈酚-3-O-β-D-吡喃葡萄糖苷（5）、山奈酚-3-O-β-D-吡喃葡萄糖苷（6）、槲皮素-3-O-β-D-吡喃葡萄糖苷（7）、山奈酚-3-O-α-L-鼠李吡喃糖苷基（1→6）-β-D-吡喃葡萄糖苷（8）和山奈酚-3-O-α-L-鼠李吡喃糖苷基（1→6）-β-D-吡喃葡萄糖苷（9），均为首次从该种植物中分离得到。这些化合物的结构是根据光谱研究，包括 1D 和 2D NMR 技术推断出来的。对这些化合物进行了生物活性研究，结果表明它们在卡拉胶诱导的大鼠足肿胀试验中具有显著的抗炎活性。通过 DPPH 自由基清除试验评价了分离得到的化合物 1-9 的抗氧化活性，与标准 BHA 相比，化合物 1、2、4 和 7-9 表现出显著的清除活性。进一步研究了这些化合物的抗糖化性质，发现一些化合物具有显著的抗糖化抑制活性。用 HPLC 法对化合物 2-5、8 和 9 的纯度进行了确认。还讨论了这些结果对 Cordia 属化学分类学研究的意义。
