Rodrigues Winner Duque, Cardoso Felipe Nunes, Baviera Amanda Martins, Dos Santos André Gonzaga
Department of Drugs and Medicines, School of Pharmaceutical Sciences, São Paulo State University, Araraquara 14800-903, Brazil.
Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University, Araraquara 14800-903, Brazil.
Antioxidants (Basel). 2023 Feb 19;12(2):522. doi: 10.3390/antiox12020522.
Jacq. (Boraginaceae) is traditionally used in the treatment of inflammatory processes. The ethanolic extract of its leaves (EEVc) showed anti-inflammatory properties and low toxicity. Medicinal plants have aroused interest for their antiglycation activities. The formation and accumulation of advanced glycation end products (AGEs) are associated with several chronic diseases. The objective of this study was to evaluate the antiglycation potential of EEVc and two isolated compounds.
The compounds brickellin and cordialin A were obtained by chromatographic methods and identified by spectrometric techniques. Analysis of fluorescent AGEs, biomarkers of amino acid residue oxidation, protein carbonyl groups and crosslink formation were performed in samples obtained from an in vitro model system of protein glycation with methylglyoxal.
EEVc, brickellin and cordialin A significantly reduced the in vitro formation of AGEs, and reduced the damage caused by oxidative damage to the protein.
According to the results, EEVc, brickellin and cordialin A are potential candidates against AGEs formation, which opens the way to expand the therapeutic arsenal for many pathologies resulting from glycoxidative stress.
紫草科植物紫草（Jacq.）传统上用于治疗炎症过程。其叶乙醇提取物（EEVc）具有抗炎特性且毒性低。药用植物因其抗糖化活性而引起了人们的兴趣。晚期糖基化终产物（AGEs）的形成和积累与多种慢性疾病有关。本研究的目的是评估EEVc和两种分离化合物的抗糖化潜力。
通过色谱法获得化合物紫草素和cordialin A，并通过光谱技术进行鉴定。在由甲基乙二醛构建的蛋白质糖基化体外模型系统所获样品中，对荧光AGEs、氨基酸残基氧化生物标志物、蛋白质羰基基团和交联形成进行了分析。
EEVc、紫草素和cordialin A显著减少了体外AGEs的形成，并减少了氧化损伤对蛋白质造成的损害。
根据结果，EEVc、紫草素和cordialin A是抗AGEs形成的潜在候选物，这为扩展针对许多由糖氧化应激导致的病症的治疗手段开辟了道路。
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