Log T, Chang K S
J Immunol Methods. 1979;26(3):291-303. doi: 10.1016/0022-1759(79)90254-0.
An enzyme immunoassay (EIA) for FOCMA has been developed. The assay uses alkaline phosphatase-conjugated rabbit anti-cat IgG as the second antibody and p-nitrophenyl phosphate as the substrate for the enzyme to detect cat FOCMA antibody bound to the target cells. In comparison with the indirect immunofluorescence (IIF) test, which was originally used for FOCMA assay, our results showed a good correlation between the two methods. The EIA gives a more objective measure of FOCMA reactivity than does IIF. FOCMA was successfully extracted from FOCMA-positive cell membranes by 0.5% Triton X-100 and further fractionated by ammonium sulfate. The FOCMA activity was assayed by IIF and EIA inhibition test. Most of the FOCMA activity was found in the fractions precipitated by 30% and 50% ammonium sulfate saturation.
已开发出一种用于检测猫胎儿心肌抗原（FOCMA）的酶免疫测定法（EIA）。该测定法使用碱性磷酸酶偶联的兔抗猫IgG作为二抗，对硝基苯磷酸作为酶的底物，以检测与靶细胞结合的猫FOCMA抗体。与最初用于FOCMA测定的间接免疫荧光（IIF）试验相比，我们的结果表明这两种方法之间具有良好的相关性。与IIF相比，EIA能更客观地衡量FOCMA反应性。通过0.5% Triton X-100成功从FOCMA阳性细胞膜中提取出FOCMA，并进一步用硫酸铵分级分离。通过IIF和EIA抑制试验测定FOCMA活性。大部分FOCMA活性存在于硫酸铵饱和度为30%和50%时沉淀的组分中。
