Rostami Nejad M, Taghipour N, Nochi Z, Mojarad E Nazemalhosseini, Mohebbi S R, Harandi M Fasihi, Zali M R
Research Center of Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Science, Tehran, Iran.
J Helminthol. 2012 Dec;86(4):485-92. doi: 10.1017/S0022149X1100071X. Epub 2011 Dec 14.
Mitochondrial genes have more power than nuclear genes in reconstructing phylogenetic relationships among closely related species because of their faster sequence evolution. The aim of this study was to use the complete or near-complete sequences from three mitochondrial genes (cox1, nad1 and atp6) and partial sequences of the 12S rRNA gene to infer relationships among isolates of Echinococcus granulosus from Iran. Two hundred and twenty-nine isolates of E. granulosus were collected from cattle, camels, sheep, buffalo and goats from different geographical areas. Most individuals were found to possess the G1 genotype but some of the camel samples belonged to the G6 genotype. Newly designed primers for cox1, nad1 and atp6 genes amplified bands of 1830, 708 and 1157 bp for the G1 genotype and 1856, 705, 1054 bp for the G6 genotype, respectively. The result of this survey showed that atp6 and nad1 genes are good molecular markers for identifying E. granulosus isolates from a range of hosts in Iran.
由于线粒体基因的序列进化更快，在重建近缘物种之间的系统发育关系时，线粒体基因比核基因更具优势。本研究的目的是利用三个线粒体基因（cox1、nad1和atp6）的完整或近乎完整的序列以及12S rRNA基因的部分序列来推断伊朗细粒棘球绦虫分离株之间的关系。从不同地理区域的牛、骆驼、绵羊、水牛和山羊中收集了229株细粒棘球绦虫分离株。发现大多数个体具有G1基因型，但一些骆驼样本属于G6基因型。新设计的cox1、nad1和atp6基因引物分别扩增出G1基因型的1830、708和1157 bp条带以及G6基因型的1856、705、1054 bp条带。本次调查结果表明，atp6和nad1基因是鉴定伊朗不同宿主来源的细粒棘球绦虫分离株的良好分子标记。
