Department of Biology, College of Science, Jouf University, Sakaka, Saudi Arabia.
Open Vet J. 2024 Mar;14(3):866-878. doi: 10.5455/OVJ.2024.v14.i3.14. Epub 2024 Mar 31.
Cystic echinococcosis (CE), which is triggered by the parasite , is a global zoonotic disease that is common in rural regions in which there are frequent encounters between dogs and other domestic animals. The disease can have devastating consequences, impacting the health of people and animals and leading to huge financial losses, especially in the agricultural industry. In the Kingdom of Saudi Arabia (KSA) and Egypt, despite the high incidence of disease, few investigations have been conducted into the genetic variation in species of the genus .
This study sought to compare the genetic features of the hydatid cysts carried in sheep in KSA with those found in Egypt.
DNA from the protoscolices was used in a PCR targeting the mitochondrial NADH dehydrogenase 1 (), cytochrome c oxidase subunit 1 (), and nuclear actin II () genes, and the resulting amplification products of 30 KSA and Egyptian isolates were sequenced and compared.
Among the sheep in KSA, the overall prevalence of CE was 0.51%. Of the sheep cyst DNA samples, 95%, 100%, and 52% were positive for the , and genes, respectively. Targeting all three genes, all KSA samples belonged to the genotype (G1), whereas all Egyptian isolates belonged to (G1) and (G6).
We conclude that isolates of from the two countries shared a common origin in Arabic North Africa, with sheep and camels as common hosts.
由寄生虫引起的包虫病（CE）是一种全球性的人畜共患病，在经常有狗和其他家畜接触的农村地区很常见。这种疾病可能会造成毁灭性的后果，影响人类和动物的健康，并导致巨大的经济损失，特别是在农业行业。在沙特阿拉伯王国（KSA）和埃及，尽管疾病发病率很高，但对属种的遗传变异的研究很少。
本研究旨在比较 KSA 绵羊携带的包虫囊与埃及发现的包虫囊的遗传特征。
使用针对线粒体 NADH 脱氢酶 1 ()、细胞色素 c 氧化酶亚基 1 () 和核肌动蛋白 II () 基因的 PCR 从Protoscolices 中提取 DNA，对 30 个 KSA 和埃及分离株的扩增产物进行测序和比较。
在 KSA 的绵羊中，CE 的总体流行率为 0.51%。在绵羊囊 DNA 样本中，95%、100%和 52%分别对基因、和呈阳性。针对所有三个基因，所有 KSA 样本均属于基因型（G1），而所有埃及分离株均属于（G1）和（G6）。
我们得出结论，来自这两个国家的包虫属分离株起源于阿拉伯北非，绵羊和骆驼是共同的宿主。
