Department of Pathology, College of Veterinary Medicine, King Faisal University, Hofof, Saudi Arabia.
Department of Parasitology and Medical Entomology, Nile University, Khartoum, Sudan.
Parasitol Res. 2021 Jun;120(6):2077-2086. doi: 10.1007/s00436-021-07156-1. Epub 2021 Apr 17.
We report on the genetic identity of 36 Echinococcus cysts that were collected during a recent slaughterhouse survey of 810 locally bred camels (dromedaries) in the Eastern Province of the Kingdom of Saudi Arabia. Analysis of a partial nad1 gene sequence showed that the majority (n = 29) belonged to E. granulosus sensu stricto, four to E. canadensis G6/7, and three to E. ortleppi. Eight of the 29 E. granulosus s.s. cysts contained protoscoleces; all other cysts were calcified and non-viable. This is the first report of the presence E. ortleppi from the Arabian Peninsula, a parasite that is typically transmitted via cattle. The results indicate widespread infection of camels with CE in eastern Saudi Arabia and an active role of camels in the lifecycles of at least E. granulosus s.s.. Complete cox1 haplotype analysis of 21 E. granulosus s.s. isolates shows that the majority of variants circulating in eastern Saudi Arabia is distinct from but closely related to haplotypes from neighboring countries in the Middle East, which indicates the presence of this parasite in KSA for a longer period of time. All isolates of E. granulosus s.s. in this study belonged to the G1 cluster, although the G3 genotype has previously also been reported from the Middle East.
我们报告了在沙特阿拉伯东部省份最近的一次屠宰场调查中，从 810 头本地饲养的骆驼（单峰骆驼）中收集的 36 个包虫囊的遗传特征。对部分 nad1 基因序列的分析表明，大多数（n=29）属于细粒棘球绦虫 E. granulosus sensu stricto，4 个属于 E. canadensis G6/7，3 个属于 E. ortleppi。29 个 E. granulosus s.s. 包虫囊中，有 8 个含有原头节；所有其他包虫囊均已钙化且无活力。这是首次从阿拉伯半岛报告存在 E. ortleppi，这是一种通常通过牛传播的寄生虫。结果表明，沙特阿拉伯东部地区的骆驼广泛感染了 CE，骆驼在至少 E. granulosus s.s. 的生命周期中发挥了积极作用。对 21 个 E. granulosus s.s. 分离株的完整 cox1 单倍型分析表明，在沙特阿拉伯东部流行的大多数变体与中东邻国的变体不同但密切相关，这表明这种寄生虫在沙特阿拉伯存在了较长时间。本研究中的所有 E. granulosus s.s. 分离株均属于 G1 群，尽管之前也曾从中东报道过 G3 基因型。
