Duarte Sabrina Castilho, Parente Juliana Alves, Pereira Maristela, Soares Célia Maria de Almeida, Linhares Guido Fontgalland Coelho
Parasitic Diseases Laboratory, Veterinary School, Federal University of Goiás, Goiás, GO, Brazil.
Rev Bras Parasitol Vet. 2011 Oct-Dec;20(4):274-80. doi: 10.1590/s1984-29612011000400004.
The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of Goiás. Only samples presenting typical intraerythrocytic parasites were used in the study. These were subjected to DNA extraction and amplification of a fragment of the 18S rRNA, by means of PCR. The PCR products were purified and sequenced. Sequences were obtained from 35 samples but only 17 of these were kept after quality assessment. Similarity analysis using BLASTn demonstrated that all 17 sequences corresponded to B. canis vogeli. Analysis using the Mega4 software showed that the isolates of B. canis vogeli from dogs in Goiânia present a high degree of molecular similarity (99.2 to 100%) in comparison with other reference isolates from other regions of Brazil and worldwide, deposited in GenBank.
巴贝斯虫属包含可引发巴贝斯虫病的原生动物。犬类通常会感染犬巴贝斯虫或吉氏巴贝斯虫。犬巴贝斯虫可分为犬巴贝斯虫犬亚种、犬巴贝斯虫韦氏亚种和犬巴贝斯虫罗氏亚种。其中，犬巴贝斯虫韦氏亚种在巴西占主导地位。本研究的目的是对来自戈亚斯州戈亚尼亚市犬只身上的巴贝斯虫分离株进行系统发育分析。从戈亚斯州一家兽医医院收治的890只出现疑似犬巴贝斯虫病临床症状的犬只身上采集血样。本研究仅使用呈现典型红细胞内寄生虫的样本。通过聚合酶链反应（PCR）对这些样本进行DNA提取和18S核糖体RNA片段的扩增。对PCR产物进行纯化和测序。从35个样本中获得了序列，但经过质量评估后仅保留了其中17个。使用BLASTn进行的相似性分析表明，所有17个序列均对应于犬巴贝斯虫韦氏亚种。使用Mega4软件进行的分析表明，与保存在GenBank中的巴西其他地区和全球其他地区的其他参考分离株相比，来自戈亚尼亚市犬只的犬巴贝斯虫韦氏亚种分离株呈现出高度的分子相似性（99.2%至100%）。
