Tayyub Muhammad, Ashraf Kamran, Lateef Muhammad, Anjum Aftab Ahmad, Ali Muhammad Asad, Ahmad Nisar, Nawaz Muhammad, Nazir Muhammad Mudasser
Department of Parasitology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Department of Microbiology, University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.
Animals (Basel). 2019 Jul 13;9(7):439. doi: 10.3390/ani9070439.
Canine babesiosis is a serious threat to dogs' health worldwide, caused by the intra-erythrocytic species. The present study was carried out in pet dogs presented at three clinics of Lahore and one of Narowal in Punjab, Pakistan. Two hundred blood samples (50 from each clinic) were collected and screened by microscopy for spp. Out of 200 samples, 84 (42%) were found to be positive for babesiosis. The highest number of positive cases (50%) was recorded in dogs at Narowal clinic. Non-significant variation ( > 0.05) was observed in the prevalence of babesiosis in dogs in relation to sex and age. Positive samples were further confirmed by a polymerase chain reaction using 18S-rRNA genus-specific and species-specific primers. Amplicons were further analyzed by nucleotide sequencing for genetic diversity. and were confirmed by genome sequencing in all diseased dogs. These isolates closely resembled each other, but differed from previous reported strains. In conclusion, pet dogs suffering from babesiosis were infected with and , while in other countries, other species are also prevalent.
犬巴贝斯虫病对全球犬类健康构成严重威胁，由红细胞内的物种引起。本研究在巴基斯坦旁遮普省拉合尔的三家诊所和纳罗瓦尔的一家诊所就诊的宠物犬中进行。采集了200份血样（每个诊所50份），并通过显微镜检查筛选 属物种。在200份样本中，发现84份（42%）巴贝斯虫病呈阳性。纳罗瓦尔诊所的犬类中阳性病例数最高（50%）。在犬类巴贝斯虫病的患病率方面，未观察到与性别和年龄相关的显著差异（>0.05）。使用18S - rRNA属特异性和种特异性引物通过聚合酶链反应进一步确认阳性样本。通过核苷酸测序对扩增子进行进一步分析以确定遗传多样性。通过基因组测序在所有患病犬中确认了 和 。这些分离株彼此非常相似，但与先前报道的菌株不同。总之，患有巴贝斯虫病的宠物犬感染了 和 ，而在其他国家，其他 属物种也很普遍。
