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本文引用的文献

1
MALDI-TOF MS improves routine identification of non-fermenting Gram negative isolates from cystic fibrosis patients.基质辅助激光解吸电离飞行时间质谱技术提高了囊性纤维化患者中非发酵革兰氏阴性菌的常规鉴定。
J Cyst Fibros. 2012 Jan;11(1):59-62. doi: 10.1016/j.jcf.2011.09.001. Epub 2011 Oct 2.
2
Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells.新兴呼吸道病原体 Pandoraea 属的体内毒力及其与肺上皮细胞的相互作用。
J Med Microbiol. 2011 Mar;60(Pt 3):289-299. doi: 10.1099/jmm.0.022657-0. Epub 2010 Dec 2.
3
Detection and accurate identification of new or emerging bacteria in cystic fibrosis patients.囊性纤维化患者中新发或出现细菌的检测和准确鉴定。
Clin Microbiol Infect. 2010 Jul;16(7):809-20. doi: 10.1111/j.1469-0691.2010.03236.x.
4
EuroCareCF quality assessment of diagnostic microbiology of cystic fibrosis isolates.欧洲囊性纤维化诊断微生物学质量评估。
J Clin Microbiol. 2009 Nov;47(11):3435-8. doi: 10.1128/JCM.01182-09. Epub 2009 Sep 9.
5
Misidentification of Pandoraea sputorum isolated from sputum of a patient with cystic fibrosis and review of Pandoraea species infections in transplant patients.从一名囊性纤维化患者痰液中分离出的斯氏潘多拉菌的错误鉴定及移植患者中潘多拉菌属感染的综述
J Clin Microbiol. 2008 Sep;46(9):3165-8. doi: 10.1128/JCM.00855-08. Epub 2008 Jul 23.
6
Evaluation of in vitro virulence characteristics of the genus Pandoraea in lung epithelial cells.潘多拉菌属在肺上皮细胞中的体外毒力特性评估。
J Med Microbiol. 2008 Jan;57(Pt 1):15-20. doi: 10.1099/jmm.0.47544-0.
7
Emerging and unusual gram-negative infections in cystic fibrosis.囊性纤维化患者中出现的新型及罕见革兰氏阴性菌感染
Semin Respir Crit Care Med. 2007 Jun;28(3):312-21. doi: 10.1055/s-2007-981652.
8
Chronic colonization with Pandoraea apista in cystic fibrosis patients determined by repetitive-element-sequence PCR.通过重复元件序列PCR确定囊性纤维化患者中潘多拉阿皮斯塔的慢性定植情况。
J Clin Microbiol. 2006 Mar;44(3):833-6. doi: 10.1128/JCM.44.3.833-836.2006.
9
Pandoraea bacteremia in a cystic fibrosis patient with associated systemic illness.一名患有相关全身性疾病的囊性纤维化患者发生洋葱伯克霍尔德菌血症。
Pediatr Infect Dis J. 2004 Sep;23(9):881-2. doi: 10.1097/01.inf.0000136857.74561.3c.
10
Epidemic spread of Pandoraea apista, a new pathogen causing severe lung disease in cystic fibrosis patients.潘多拉阿皮斯塔菌在囊性纤维化患者中引发严重肺部疾病的新病原体的流行传播。
Pediatr Pulmonol. 2003 Nov;36(5):439-46. doi: 10.1002/ppul.10383.

通过结合 16S rRNA 测序和基质辅助激光解吸电离飞行时间质谱技术鉴定的慢性定植于 Pandoraea sputorum 的囊性纤维化患者的临床和微生物特征。

Clinical and microbiological features of a cystic fibrosis patient chronically colonized with Pandoraea sputorum identified by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry.

机构信息

Servicio de Microbiología and CIBER en Epidemiología y Salud Pública (CIBERESP), Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS) and Hospital Universitario Ramón y Cajal, Madrid, Spain.

出版信息

J Clin Microbiol. 2012 Mar;50(3):1096-8. doi: 10.1128/JCM.05730-11. Epub 2011 Dec 14.

DOI:10.1128/JCM.05730-11
PMID:22170922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3295115/
Abstract

Clonal isolates identified as various nonfermentative Gram-negative bacilli over a 5-year period from sputum cultures of a 30-year-old cystic fibrosis patient were successfully reidentified as Pandoraea sputorum by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Decreased lung function improved after 1 year of azithromycin and inhaled 7%-hypertonic saline treatment.

摘要

在过去的 5 年中,从一名 30 岁囊性纤维化患者的痰培养物中分离出的各种非发酵革兰氏阴性杆菌的克隆分离物,通过 16S rRNA 测序和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)相结合,成功地重新鉴定为 Pandoraea sputorum。经过 1 年的阿奇霉素和吸入 7%-高渗盐水治疗后,肺功能下降得到改善。