RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Akkermaalsbos 2, 6708WB, P.O. Box 230, 6700AE Wageningen, The Netherlands.
Anal Bioanal Chem. 2012 Feb;402(4):1611-23. doi: 10.1007/s00216-011-5581-3. Epub 2011 Dec 16.
A liquid chromatography-tandem mass spectrometry method for the analysis of seven antiviral drugs, zanamivir, ribavirin, oseltamivir, oseltamivir carboxylate, amantadine, rimantadine and arbidol, in poultry muscle is reported. The antiviral drugs were extracted from the homogenized poultry muscle sample using methanol. The extract was purified using tandem solid-phase extraction combining a cation exchange cartridge and a phenylboronic acid cartridge. To prevent excessive matrix effects, the analytes were separated from the matrix constituents using a column-switch liquid chromatography system combining a reversed-phase and a Hypercarb analytical column. Detection was carried out using tandem mass spectrometry. The method was fully validated according to 2002/657/EC [1] and proved to be adequate for quantification and confirmation of zanamivir and ribavirin at 10 μg kg(-1), oseltamivir, oseltamivir carboxylate, amantadine and rimantadine at levels below 1.0 μg kg(-1) and for qualitative confirmatory analysis of arbidol at levels below 1 μg kg(-1).
本文报道了一种用于检测禽肉中七种抗病毒药物（扎那米韦、利巴韦林、奥司他韦、奥司他韦羧酸盐、金刚烷胺、金刚烷乙胺和阿比朵尔）的液相色谱-串联质谱法。抗病毒药物从匀浆禽肉样品中用甲醇提取。提取物通过串联固相萃取（阳离子交换柱和苯硼酸柱）进行纯化。为了防止基质效应过大，使用柱切换液相色谱系统（反相和 Hypercarb 分析柱）将分析物与基质成分分离。采用串联质谱法进行检测。该方法按照 2002/657/EC[1]进行了全面验证，证明其适用于扎那米韦和利巴韦林在 10μg/kg（-1）水平、奥司他韦、奥司他韦羧酸盐、金刚烷胺和金刚烷乙胺在 1.0μg/kg（-1）以下水平的定量和确证分析，以及阿比朵尔在 1μg/kg（-1）以下水平的定性确证分析。
