Department of Radiology, Ruijin Hospital, School of Medicine, Shanghai, China.
Stroke. 2012 Mar;43(3):888-91. doi: 10.1161/STROKEAHA.111.636456. Epub 2011 Dec 15.
The intraluminal suture technique for producing middle cerebral artery occlusion in rodents is the most commonly used method for modeling focal cerebral ischemia associated with clinical ischemic stroke. Synchrotron radiation angiography may provide a novel solution to directly monitor the success of middle cerebral artery occlusion.
Twenty adult Sprague-Dawley rats for middle cerebral artery occlusion models were prepared randomly with different suture head silicone coating. In vivo imaging was performed at beam line BL13W1, Shanghai Synchrotron Radiation Facility, Shanghai, China.
Silicone-coated suture was superior to uncoated suture for producing consistent brain infarction. Additionally, silicone coating length was an important variable controlling the extent of the ischemic lesion: infarcts affected predominantly the caudate-putamen with large variability (<2 mm), both the cortex and caudate-putamen (2-3.3 mm), and most of the hemisphere, including the hypothalamus (>3.3 mm).
Synchrotron radiation angiography provides a useful tool to observe hemodynamic changes after middle cerebral artery occlusion, and the physical properties of suture are critical to the success of the middle cerebral artery occlusion model.
在啮齿动物中进行大脑中动脉闭塞的管腔内缝线技术是模拟与临床缺血性中风相关的局灶性脑缺血的最常用方法。同步辐射血管造影术可能为直接监测大脑中动脉闭塞的成功提供一种新的解决方案。
随机使用不同的缝线头部硅酮涂层准备了 20 只用于大脑中动脉闭塞模型的成年 Sprague-Dawley 大鼠。在上海同步辐射装置的 BL13W1 光束线上进行了体内成像。
硅酮涂层缝线比未涂层缝线更能产生一致的脑梗死。此外，硅酮涂层长度是控制缺血性病变程度的一个重要变量：梗塞主要影响尾状核-壳核，变异性较大（<2mm），同时影响皮质和尾状核-壳核（2-3.3mm），以及包括下丘脑在内的大部分半球（>3.3mm）。
同步辐射血管造影术为观察大脑中动脉闭塞后的血液动力学变化提供了一种有用的工具，缝线的物理特性对大脑中动脉闭塞模型的成功至关重要。
