Loma Linda University School of Medicine, Loma Linda, CA 92354, USA.
Br J Dermatol. 2012 Apr;166(4):761-70. doi: 10.1111/j.1365-2133.2011.10781.x. Epub 2012 Mar 12.
p-tert-Butylphenol-formaldehyde resin (PTBP-FR) is a common component of glues used in the manufacturing of many plastic, electronic, rubber, wood and leather products. Two main allergens of PTBP-FR have been described.
To determine the concentrations of the two main allergens of PTBP-FR in diagnostic patch testing preparations and PTBP-FR available to glue and adhesive manufacturers.
Nuclear magnetic resonance spectrometry was used to confirm the identity and determine the purity of reference materials. High-pressure liquid chromatography was used to analyse patch test preparations and commercially available PTBP-FR.
In the PTBP-FR in analysed patch test preparations the highest concentration of the allergenic dimer 4-tert-butyl-2-(5-tert-butyl-2-hydroxy-3-hydroxymethyl-benzyloxymethyl)-6-hydroxymethylphenol found was 1·79% and the lowest 0·21%. The highest concentration of the allergenic dimer 4-tert-butyl-2-(5-tert-butyl-2-hydroxy-benzyloxymethyl)-6-hydroxymethylphenol found in PTBP-FR of analysed patch test preparations was 0·50% and the lowest concentration found was 0·04%. In commercially available PTBP-FR the highest concentration of 4-tert-butyl-2-(5-tert-butyl-2-hydroxy-3-hydroxymethyl-benzyloxymethyl)-6-hydroxymethylphenol found was 3·7% and the highest concentration of 4-tert-butyl-2-(5-tert-butyl-2-hydroxy-benzyloxymethyl)-6-hydroxymethylphenol found was 1·1%. In three PTBP-FR samples neither allergen could be detected.
Our data suggest that reporting resin concentration in petrolatum is not predictive of a consistent concentration of the two main allergens of PTBP-FR. The 10-fold difference in allergen concentration between different patch test preparations has significant ramifications for maintaining consistent dose of delivered allergen. The results of this study reinforce the need for patch test product standardization in the contact dermatitis community.
对叔丁基苯酚甲醛树脂（PTBP-FR）是许多塑料、电子、橡胶、木材和皮革产品制造中使用的胶水的常见成分。已经描述了 PTBP-FR 的两种主要过敏原。
确定诊断斑贴试验制剂和胶黏剂制造商可用的 PTBP-FR 中两种主要过敏原的浓度。
使用核磁共振光谱法确认参考物质的身份和纯度。使用高压液相色谱法分析斑贴试验制剂和市售的 PTBP-FR。
在所分析的斑贴试验制剂中的 PTBP-FR 中，发现致敏二聚体 4-叔丁基-2-(5-叔丁基-2-羟基-3-羟甲基-苯甲氧基甲基)-6-羟甲基苯酚的最高浓度为 1.79%，最低浓度为 0.21%。在所分析的斑贴试验制剂中的 PTBP-FR 中发现致敏二聚体 4-叔丁基-2-(5-叔丁基-2-羟基苯甲氧基甲基)-6-羟甲基苯酚的最高浓度为 0.50%，最低浓度为 0.04%。在市售的 PTBP-FR 中，发现 4-叔丁基-2-(5-叔丁基-2-羟基-3-羟甲基-苯甲氧基甲基)-6-羟甲基苯酚的最高浓度为 3.7%，发现 4-叔丁基-2-(5-叔丁基-2-羟基苯甲氧基甲基)-6-羟甲基苯酚的最高浓度为 1.1%。在三个 PTBP-FR 样品中均未检测到这两种过敏原。
我们的数据表明，报告凡士林中的树脂浓度并不能预测 PTBP-FR 的两种主要过敏原的一致浓度。不同斑贴试验制剂中过敏原浓度相差 10 倍，这对保持一致的过敏原剂量有重大影响。本研究的结果强化了接触性皮炎社区中斑贴试验产品标准化的必要性。
