Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
Molecules. 2011 Dec 19;16(12):10631-44. doi: 10.3390/molecules161210631.
The effects of the oligosaccharides from the endophytic fungus Fusarium oxysporum Dzf17 as elicitors on diosgenin production in cell suspension cultures of its host Dioscorea zingiberensis were investigated. Three oligosaccharides, DP4, DP7 and DP10, were purified from the oligosaccharide fractions DP2-5, DP5-8 and DP8-12, respectively, which were prepared from the water-extracted mycelial polysaccharide of the endophytic fungus F. oxysporum Dzf17. When the cell cultures were treated with fraction DP5-8 at 20 mg/L on day 26 and harvested on day 32, the maximum diosgenin yield (2.187 mg/L) was achieved, which was 5.65-fold of control (0.387 mg/L). When oligosaccharides DP4, DP7 and DP10 were individually added to 26-day-old D. zingiberensis cell cultures at concentrations of 2, 4, 6, 8 and 10 mg/L in medium, DP7 at 6 mg/L was found to significantly enhance diosgenin production, with a yield of 3.202 mg/L, which was 8.27-fold of control. When the cell cultures were treated with DP7 twice on days 24 and 26, and harvested on day 30, both diosgenin content and yield were significantly increased and reached the maximums of 1.159 mg/g dw and 4.843 mg/L, both of which were higher than those of single elicitation, and were 9.19- and 12.38-fold of control, respectively.
研究了内生真菌尖孢镰刀菌 Dzf17 的寡糖作为诱导子对其宿主盾叶薯蓣细胞悬浮培养物中薯蓣皂苷元生产的影响。从内生真菌尖孢镰刀菌 Dzf17 的水提菌丝体多糖中分别制备了寡糖 DP2-5、DP5-8 和 DP8-12 ，并从这些寡糖中纯化得到三种寡糖 DP4、DP7 和 DP10。当细胞培养物在第 26 天用 20mg/L 的 DP5-8 处理并在第 32 天收获时，薯蓣皂苷元的最大产量（2.187mg/L）达到了对照（0.387mg/L）的 5.65 倍。当 DP4、DP7 和 DP10 这三种寡糖分别以 2、4、6、8 和 10mg/L 的浓度添加到 26 天龄的盾叶薯蓣细胞培养物中时，发现 DP7 在 6mg/L 时能显著提高薯蓣皂苷元的产量，达到 3.202mg/L，是对照的 8.27 倍。当细胞培养物在第 24 天和第 26 天分别用 DP7 处理两次，并在第 30 天收获时，薯蓣皂苷元的含量和产量均显著增加，达到了 1.159mg/gdw 和 4.843mg/L 的最大值，均高于单次诱导，分别是对照的 9.19 倍和 12.38 倍。
