Jasim B, Thomas Roshmi, Mathew Jyothis, Radhakrishnan E K
School of Biosciences, Mahatma Gandhi University, PD Hills (PO), Kottayam, Kerala 686 560, India.
Saudi Pharm J. 2017 Mar;25(3):443-447. doi: 10.1016/j.jsps.2016.09.012. Epub 2016 Sep 30.
Various methods have been used to enhance production of chemically diverse phytochemicals especially medicinal natural products. With the advancement in nanotechnology, nanoparticles have been reported to have varying impact in plant growth and inducibility of phytochemical composition. Major objective of the study was to study the secondary metabolite modulatory effect of silver nanoparticles. In the current study, treatment of fenugreek seedlings with biosynthesized silver nanoparticles (Ag-NPs) was found to have significant impact on its growth parameters such as leaf number, root length, shoot length and wet weight. On HPLC based analysis, Ag-NPs treated seedlings showed an enhancement in the production of major phytochemical diosgenin to a level of 214.06 ± 17.07 μg/mL. An untreated control gave an yield of only 164.44 ± 7.67 μg/mL of diosgenin, and the observed phytochemical enhancement effect induced by Ag-NP was very significant. Most remarkably, the Ag-NP used in the study was found to play dual role of enhancement of both plant growth and diosgenin synthesis. Hence the study is of immense application as it opens up development of new methods based on nanoelicitors to enhance the biosynthesis of medicinal natural products in plants.
人们已经使用了各种方法来提高化学性质多样的植物化学物质，尤其是药用天然产物的产量。随着纳米技术的进步，据报道纳米颗粒对植物生长和植物化学成分的诱导性有不同影响。该研究的主要目的是研究银纳米颗粒对次生代谢物的调节作用。在当前研究中，发现用生物合成的银纳米颗粒（Ag-NPs）处理胡芦巴幼苗对其生长参数，如叶片数量、根长、茎长和湿重有显著影响。基于高效液相色谱（HPLC）的分析表明，经Ag-NPs处理的幼苗中主要植物化学物质薯蓣皂苷元的产量提高到了214.06±17.07μg/mL的水平。未经处理的对照仅产生了164.44±7.67μg/mL的薯蓣皂苷元产量，并且观察到的由Ag-NP诱导的植物化学增强效应非常显著。最值得注意的是，研究中使用的Ag-NP被发现对植物生长和薯蓣皂苷元合成均具有增强的双重作用。因此，该研究具有巨大的应用价值，因为它开启了基于纳米激发子的新方法的开发，以增强植物中药用天然产物的生物合成。
