Hassan H, Shorman M
Department of Pathology and Laboratory Medicine, King Fahad Specialist Hospital-Dammam (KFSHD), Dammam 31444, Saudi Arabia.
Int J Microbiol. 2011;2011:861514. doi: 10.1155/2011/861514. Epub 2011 Dec 1.
Objectives. The aim of this study was to evaluate the diagnostic performance of BD GeneOhm VanR Assay, a rapid PCR test that detects the presence of vanA and/or vanB genes and the performance of BDGeneOhm MRSA Assay which detects the staphylococcal cassette chromosomemec (SCCmec cassette) carrying the mecA gene and Staphylococcus aureus specific sequence located within the orfX gene. Methods. 300 duplicate rectal swabs collected consecutively were analyzed for the presence of VRE by culture and BD PCR. 2267 duplicate swabs were collected (728 nasal and 1539 groin swabs) and analyzed for the presence of MRSA by culture method and BD PCR. Results. Compared to culture, the BD GeneOhm VanR Assay showed a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 100%, 91.1%, 23.5%, and 100%, respectively. The BD GeneOhm MRSA Assay revealed sensitivity, specificity, PPV, and NPV of 97.2%, 99.4%, 89.7%, and 99.9%, respectively, for nasal swabs. For groin swabs, it was 100%, 98.7%, 61.5% and 100%, respectively. Conclusion. The BD GeneOhm vanR Assay is a good screening test for rapid exclusion of VRE carriers in hospitals. The BD GeneOhm MRSA Assay represents a reliable screening test. The true strength of the BD GeneOhm Assay for MRSA and VRE is its exceptionally high NPV making the test an ideal tool for rapid exclusion of MRSA and VRE carriers in hospitals. As a consequence, this would dramatically shorten the patient isolation time.
目的。本研究旨在评估BD GeneOhm VanR检测法的诊断性能，这是一种快速聚合酶链反应（PCR）检测方法，可检测vanA和/或vanB基因的存在，同时评估BD GeneOhm MRSA检测法的性能，该检测法可检测携带mecA基因的葡萄球菌盒式染色体mec（SCCmec盒式结构）以及位于orfX基因内的金黄色葡萄球菌特异性序列。方法。对连续收集的300份重复直肠拭子进行培养和BD PCR分析，以检测耐万古霉素肠球菌（VRE）的存在。收集了2267份重复拭子（728份鼻拭子和1539份腹股沟拭子），并通过培养方法和BD PCR分析耐甲氧西林金黄色葡萄球菌（MRSA）的存在。结果。与培养法相比，BD GeneOhm VanR检测法的灵敏度、特异性、阳性预测值（PPV）和阴性预测值（NPV）分别为100%、91.1%、23.5%和100%。BD GeneOhm MRSA检测法对鼻拭子的灵敏度、特异性、PPV和NPV分别为97.2%、99.4%、89.7%和99.9%。对于腹股沟拭子，其灵敏度、特异性、PPV和NPV分别为100%、98.7%、61.5%和100%。结论。BD GeneOhm vanR检测法是医院中快速排除VRE携带者 的良好筛查试验。BD GeneOhm MRSA检测法是一种可靠的筛查试验。BD GeneOhm检测法对MRSA和VRE的真正优势在于其极高的NPV，使其成为医院中快速排除MRSA和VRE携带者的理想工具。因此，这将大大缩短患者的隔离时间。
