Department of Food Science, University of Guelph, Guelph, ON, Canada.
J Nutr. 2012 Feb;142(2):363-8. doi: 10.3945/jn.111.149104. Epub 2011 Dec 21.
We evaluated the antiinflammatory activity of soy-derived di- and tripeptides in a dextran sodium sulfate (DSS)-induced pig model of intestinal inflammation. In the DSS-positive control (POS) and DSS-positive with soy peptide treatment (SOY) groups (n = 6/group), DSS was administered to piglets via i.g. catheter for 5 d, followed by a 5-d administration of saline or soy-derived peptides, respectively. A negative control (NEG) group received saline in lieu of the DSS and soy peptides. The severity of inflammation was assessed by clinical signs, morphological and histological measurements, gut permeability, and neutrophil infiltration. Local production of TNF and IL6 were measured by ELISA, colonic and ileal inflammatory gene expression were assessed by real-time RT-PCR, and CD4+CD25+ lymphocyte populations were analyzed by flow cytometry. Crypt elongation and muscle thickness, d-mannitol gut permeation, colonic expression of the inflammatory mediators IFNG, IL1B, TNF, RORC, and IL17A as well as the FOXP3 T-regulatory transcription factor, and myeloperoxidase activity were lower (P < 0.05) in the SOY pigs than in POS pigs. Messenger RNA levels of ileal IFNG, TNF, IL12B, and IL17A were lower (P < 0.05) and FOXP3 expression was greater (P < 0.05) in SOY piglets than in the POS group. In the mesenteric lymph nodes, CD4+CD25+ T cells were higher (P < 0.05) in both the POS and SOY groups than in NEG controls. Soy-derived peptides exert antiinflammatory activity in vivo, suggesting their usefulness for the treatment of inflammatory disorders.
我们评估了大豆衍生的二肽和三肽在葡聚糖硫酸钠（DSS）诱导的猪肠道炎症模型中的抗炎活性。在 DSS 阳性对照组（POS）和 DSS 阳性加大豆肽处理组（SOY）（每组 6 只）中，通过口服导管给仔猪施用 DSS 5 天，随后分别施用生理盐水或大豆衍生肽 5 天。阴性对照组（NEG）用生理盐水代替 DSS 和大豆肽。通过临床症状、形态学和组织学测量、肠道通透性和中性粒细胞浸润来评估炎症的严重程度。通过 ELISA 测量 TNF 和 IL6 的局部产生，通过实时 RT-PCR 评估结肠和回肠的炎症基因表达，并通过流式细胞术分析 CD4+CD25+淋巴细胞群。在 SOY 猪中，隐窝伸长和肌肉厚度、d-甘露醇肠道通透性、结肠中炎症介质 IFNG、IL1B、TNF、RORC 和 IL17A 以及 FOXP3 T 调节转录因子的表达以及髓过氧化物酶活性均低于 POS 猪（P<0.05）。与 POS 组相比，SOY 仔猪回肠中 IFNG、TNF、IL12B 和 IL17A 的信使 RNA 水平较低（P<0.05），FOXP3 表达较高（P<0.05）。在肠系膜淋巴结中，POS 和 SOY 组的 CD4+CD25+T 细胞均高于 NEG 对照组（P<0.05）。大豆衍生肽在体内具有抗炎活性，表明其在治疗炎症性疾病方面具有用途。
