Roberts J L, Seeburg P H, Shine J, Herbert E, Baxter J D, Goodman H M
Proc Natl Acad Sci U S A. 1979 May;76(5):2153-7. doi: 10.1073/pnas.76.5.2153.
A cDNA fragment synthesized from mouse mRNA (ACTH/LPH mRNA) that codes for the precursor polypeptide containing corticotropin (ACTH), beta-lipotropin (LPH), and several other peptides has been cloned in bacteria. The mRNA was enriched for ACTH/LPH mRNA translational activity (to about 75%) prior to cDNA synthesis. It appears to contain about 1200 bases, of which approximately 450 bases are not translated. The cloned DNA fragment is complementary to the region of the mRNA coding for the protein fragment beta-LPH-(44--90); this contains all of the amino acids of [Met]-enkephalin (residues 61--65 of beta-LPH), most of the amino acids of beta-melanocyte-stimulating hormone, and all but the carboxy-terminal amino acid of beta-endorphin. Based on assignment of the amino acid sequence of mouse beta-LPH from the nucelic acid sequence, it appears that there is extensive homology of mouse beta-LPH with human and porcine beta-LPH. The data also establish the linkage between beta-melanocyte-stimulating hormone and beta-endorphin as a Lys-Arg sequence. It is hoped that this cloned DNA can be used as a probe to study the expression and structure of the ACTH/LPH gene.
从小鼠信使核糖核酸（促肾上腺皮质激素/β-促脂素信使核糖核酸，ACTH/LPH mRNA）合成的互补脱氧核糖核酸（cDNA）片段，该信使核糖核酸编码含促肾上腺皮质激素（ACTH）、β-促脂素（β-LPH）和其他几种肽的前体多肽，已在细菌中克隆。在合成cDNA之前，该信使核糖核酸的ACTH/LPH信使核糖核酸翻译活性得到了富集（至约75%）。它似乎含有约1200个碱基，其中约450个碱基未被翻译。克隆的DNA片段与编码蛋白质片段β-LPH-(44--90)的信使核糖核酸区域互补；这包含了[甲硫氨酸]-脑啡肽的所有氨基酸（β-LPH的61--65位残基）、β-黑素细胞刺激素的大部分氨基酸以及β-内啡肽除羧基末端氨基酸外的所有氨基酸。根据从核酸序列推导的小鼠β-LPH氨基酸序列，小鼠β-LPH与人及猪的β-LPH似乎有广泛的同源性。这些数据还确定了β-黑素细胞刺激素与β-内啡肽之间以赖氨酸-精氨酸序列相连。希望这个克隆的DNA能用作探针来研究ACTH/LPH基因的表达和结构。
