Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya University, Higashi-ku, Nagoya, Japan.
Biomaterials. 2010 May;31(14):4094-103. doi: 10.1016/j.biomaterials.2010.01.134. Epub 2010 Feb 19.
Quantum dots (QDs) have been used to study the effects of fluorescent probes for biomolecules and cell imaging. Adipose tissue-derived stem cells, which carry a relatively lower donor site morbidity, while yielding a large number of stem cells at harvest, were transduced with QDs using the octa-arginine peptide (R8) cell-penetrating peptide (CPP). The concentration ratio of QDs:R8 of 1 x 10(4) was optimal for delivery into ASCs. No cytotoxicity was observed in ASCs transduced with less than 16 nM of QDs655. In addition, >80% of the cells could be labeled within 1 h and the fluorescent intensity was maintained at least for 2 weeks. The ASCs transduced with QDs using R8 could be differentiated into both adipogenic and osteogenic cells, thus suggesting that the cells maintained their stem cell potency. The ASCs labeled with QDs using R8 were further transplanted subcutaneously into the backs of mice or into mice through the tail vein. The labeled ASCs could be imaged with good contrast using the Maestro in vivo imaging system. These data suggested that QD labeling using R8 could be utilized for the imaging of ASCs.
量子点 (QDs) 已被用于研究生物分子和细胞成像的荧光探针的效果。脂肪组织来源的干细胞，其携带相对较低的供体部位发病率，而在收获时产生大量的干细胞，使用八精氨酸肽 (R8) 细胞穿透肽 (CPP) 将 QDs 转导到 ASCs 中。QD:R8 的浓度比为 1 x 10(4) 是将其递送到 ASCs 中的最佳浓度。小于 16 nM 的 QDs655 转导的 ASCs 中未观察到细胞毒性。此外，在 1 小时内可以标记超过 80%的细胞，并且荧光强度至少维持 2 周。使用 R8 将 QDs 转导的 ASCs 可以分化为成脂和成骨细胞，因此表明细胞保持其干细胞潜能。使用 R8 将 QDs 标记的 ASCs 进一步通过皮下或尾静脉移植到小鼠背部。使用 Maestro 体内成像系统可以很好地对比成像标记的 ASCs。这些数据表明，使用 R8 进行 QD 标记可用于 ASCs 的成像。
