Department of Energy Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan.
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Nagoya, Japan.
Cell Transplant. 2023 Jan-Dec;32:9636897231176442. doi: 10.1177/09636897231176442.
Stem cell therapy plays an important role in regenerative therapy; however, there is little information on the dynamics of transplanted stem cells and the influence of the inflammation of affected tissues or organs on these dynamics. In this study, we revealed real-time dynamics of transplanted adipose tissue-derived stem cells (ASCs) and the influence of the inflammatory states on these dynamics in acute liver failure mice. Quantum dots (QDs) labeling did not affect the cytokine profile of ASCs, and intravenously transplanted ASCs labeled with QDs could be detected in real time with high efficiency without laparotomy. Until 30 min after ASC transplantation, no marked differences in the behavior or accumulation of transplanted ASCs in the liver were observed among the three groups with different degrees of liver damage (normal, weak, and strong). However, significant differences in the engraftment rate of transplanted ASCs in the liver were observed among the three groups from 4 h after transplantation. The engraftment rate was inversely correlated with the extent of the liver damage. These data suggested that QDs are useful for real-time imaging of transplanted cells, and the inflammatory state of tissues or organs may affect the engraftment rate of transplanted cells.
干细胞治疗在再生治疗中起着重要作用；然而，关于移植干细胞的动力学以及受影响组织或器官的炎症对这些动力学的影响的信息很少。在这项研究中，我们揭示了急性肝衰竭小鼠中移植脂肪组织来源的干细胞（ASCs）的实时动力学以及炎症状态对这些动力学的影响。量子点（QD）标记不会影响 ASCs 的细胞因子谱，并且可以通过非手术方式实时高效地检测到用 QD 标记的静脉内移植的 ASCs。直到 ASC 移植后 30 分钟，在具有不同程度肝损伤（正常、弱和强）的三组中，移植的 ASCs 在肝脏中的行为或积累没有明显差异。然而，从移植后 4 小时开始，三组之间观察到移植的 ASCs 在肝脏中的植入率存在显著差异。植入率与肝损伤的程度呈负相关。这些数据表明 QD 可用于实时成像移植细胞，并且组织或器官的炎症状态可能会影响移植细胞的植入率。
